氯化胆碱/乳酸低共熔溶剂预处理对笋壳物化性质及酶解效率的影响  

Effects of choline chloride/lactic acid deep eutectic solvent pretreatment on the physicochemical properties and enzymatic hydrolysis efficiency of bamboo shoot shell

作　　者：胡强 王延云[2] 蒋琼[2] 龚卫华 王燕 HU Qiang;WANG Yanyun;JIANG Qiong;GONG Weihua;WANG Yan(Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province,Leshan Normal University,Leshan 614000,China;College of Life Science,Leshan Normal University,Leshan 614000,China;National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides,Jishou University,Jishou 416000,China)

机构地区：[1]乐山师范学院竹类病虫防控与资源开发四川省重点实验室,四川乐山614000 [2]乐山师范学院生命科学学院,四川乐山614000 [3]吉首大学杜仲综合利用技术国家地方联合工程实验室,湖南吉首416000

出　　处：《食品与发酵工业》2022年第23期187-193,共7页Food and Fermentation Industries

基　　金：乐山市科技局重点研究项目(20NZD006,21NZD044);乐山师范学院“科研培育计划项目”(2022SSDJS002)。

摘　　要：笋壳是竹笋加工过程中的副产物,为解决笋壳生物质的转化利用,其关键的一步是建立一种绿色、高效的提高笋壳酶水解效率的预处理方法。以笋壳为原料,采用不同条件的低共熔溶剂(deep-eutectic solvents,DES)预处理,利用扫描电镜、X-射线衍射及傅里叶红外光谱等手段分析笋壳木质纤维素成分及物化特征的变化,探究DES预处理方法在笋壳样品纤维素酶水解效率方面提升的效果和潜在原因。结果表明,DES预处理后,笋壳样品中纤维素的含量从35.86%上升至48.63%,半纤维素的含量从19.13%下降到15.59%,木质素的含量从28.67%下降到14.82%;DES预处理有效地破坏了笋壳木质纤维素致密的外观形态,并较大程度地减小了笋壳的抗降解屏障。数据表明,DES较大程度地减少笋壳中的木质素和少量的半纤维素,而高度保留了纤维素且DES预处理并未改变笋壳纤维素的晶型结构;笋壳样品的酶水解率从29.24%(未处理)提升至90.43%(110℃,氯化胆碱和乳酸摩尔比为1∶9),比原材料的酶水解率提升了3.1倍。研究显示,氯化胆碱/乳酸在最佳条件中(110℃,摩尔比1∶9)能实现笋壳高效酶解转化,且能实现笋壳木质素的有效脱除。Bamboo shoot husk is a by-product OF bamboo shoot processing.In order to solve the conversion and utilization of bamboo shoot husk biomass,the key step is to establish a green and efficient pretreatment method to improve the enzymatic hydrolysis efficiency of bamboo shoot husk.In this study,bamboo shoot shell was used as raw material and pretreated with deep eutectic solvent(DES)under different conditions.Scanning electron microscopy,X-ray diffraction and Fourier transform infrared spectroscopy were used to analyze the composition and physical and chemical characteristics of lignocellulose from bamboo shoot shell.The effect and potential reasons of DES pretreatment on the improvement of cellulase hydrolysis efficiency of bamboo shoot husk samples were explored.The results showed that after DES pretreatment,the cellulose content in sand shoot shell samples increased from 35.86%to 48.63%,the hemicellulose content decreased from 19.13%to 15.59%,and the lignin content decreased from 28.67%to 14.82%.DES pretreatment can effectively destroy the dense appearance of lignocellulose and reduce the anti-degradation barrier of bamboo shoot shell to a large extent.DES greatly reduced lignin and a small amount of hemicellulose,but highly retained cellulose,and DES pretreatment did not change the crystal structure of cellulose.The enzymatic hydrolysis rate of bamboo shoot shell samples increased from 29.24%(untreated)to 90.43%(110℃,1∶9),which was 3.1 times higher than that of raw materials.In summary,choline chloride/lactic acid can achieve efficient enzymatic hydrolysis of bamboo shoot husk under the best conditions(110℃,1∶9),and effectively remove the lignin in bamboo shoot husk.

关 键 词：笋壳 木质纤维素 低共熔溶剂 生物质 酶水解 

分 类 号：TS209[轻工技术与工程—食品科学]