EGC和EGCG降低α_(s1)-酪蛋白致敏小鼠过敏反应  

EGC and EGCG Reducing the Allergic Reaction of Mice Sensitized withαs1-Casein

作　　者：郭红蕾 于晓凤[1] 张倩倩 丛艳君[1] 李邻峰[2] Guo Honglei;Yu Xiaofeng;Zhang Qianqian;Cong Yanjun;Li Linfeng(Beijing Food Additive Engineering Technology Research Center,Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food and Health,Beijing Technology and Business University,Beijing 100048;Department of Dermatology,Beijing Friendship Hospital,Beijing 100050)

机构地区：[1]北京工商大学食品与健康学院北京食品营养与人类健康高精尖创新中心北京市食品添加剂工程技术研究中心,北京100048 [2]北京友谊医院皮肤科,北京100050

出　　处：《中国食品学报》2022年第11期181-189,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2019YFC1605002);国家自然科学基金项目(31872886)。

摘　　要：为研究茶多酚中的表没食子儿茶素(EGC)和表没食子儿茶素没食子酸酯(EGCG)的抗α_(s1)-酪蛋白致敏机理,首先用α_(s1)-酪蛋白致敏BALB/C小鼠,在激发阶段用EGC和EGCG给予营养干预,以降低α_(s1)-酪蛋白致敏小鼠的过敏反应。以小鼠体内肥大细胞蛋白酶含量、组胺含量、特异性抗体IgE水平、细胞因子分泌水平以及组织病理学观察为主要指标,评价EGC和EGCG降低α_(s1)-酪蛋白致敏小鼠过敏反应的能力。结果表明:EGC和EGCG均可显著降低过敏小鼠的肥大细胞蛋白酶、组胺、特异性IgE抗体和Th2型细胞因子水平。同时,组织病理学结果显示:EGC和EGCG显著降低肠、胸腺、脾脏和肺的病变程度。本研究结果可为茶多酚调控牛乳过敏原致敏反应发生的路径提供理论参考,未来可从信号转导和基因表达水平方面进一步探讨抗过敏机制。In order to study the anti-α_(s1)-casein allergy mechanism of EGC and EGCG,BALB/C mice were first sensitized with α_(s1)-casein,and nutritional intervention was given by EGC and EGCG during the challenge stage.The contents of mast cell protease,histamine,specific antibody IgE,cytokine secretion and histopathological observation were used as the main evaluation indexes.The results showed that both EGC and EGCG significantly reduced the levels of mast cell protease,histamine,specific IgE antibodies and Th2 cytokines in allergic mice.Meantime,the results about histopathology showed that EGC and EGCG significantly reduced the degree of the lesions in the intestine,thymus,spleen and lung.The conclusions can provide theoretical basis for the pathway of tea polyphenols to regulate cow milk allergen,and further studies need to be done to explore the mechanism of anti-allergy in terms of signal transduction and gene expression level.

关 键 词：α_(s1)-酪蛋白 表没食子儿茶素 表没食子儿茶素没食子酸酯 抗过敏 

分 类 号：TS201.4[轻工技术与工程—食品科学]