猴头菌与人参双向固体发酵菌质体外抗氧化活性分析  

In Vitro Antioxidant Activity Analysis of the Bi-directional Solid Fermentation Plasm of Hericium erinaceus and Ginseng

作　　者：唐敏 冷悦 王淑敏 万茜淋 王欢 陈长宝 TANG Min;LENG Yue;WANG Shumin;WAN Xilin;WANG Huan;CHEN Changbao(Jilin Ginseng Research Institute of Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学吉林省人参科学研究院,吉林长春130117

出　　处：《食品工业科技》2023年第1期154-161,共8页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目技术创新引导-医药健康产业发展专项(20210401121YY);长春中医药大学“杏林学者工程”青年科学家项目培养计划(QNKXJ2-2021ZR03)。

摘　　要：目的:为了更好地研究与利用猴头菌及人参的药用价值。方法:本文以猴头菌为发酵菌种,人参为药性基质(PGP组)进行双向固体发酵,得到猴头菌-人参双向固体发酵菌质(HEP组),通过苯酚-硫酸法、考马斯亮蓝法测定不同发酵时期的发酵菌质组及人参药性基质组的不同醇沉组分多糖及蛋白质的含量,并采用DPPH·法、·OH法、ABTS+·法测定不同醇沉组分的抗氧化能力。结果:总糖含量以40%醇沉组分最多,发酵至第9 d时,发酵菌质组40%醇沉组分HEP-40总糖含量达到0.98 mg/g,人参基质组40%醇沉组分PGP-40总糖含量达到1.03 mg/g,还原糖、蛋白质含量以90%醇沉组分的最多,发酵至第30 d时,发酵菌质组90%醇沉组分HEP-90还原糖含量达到0.18 mg/g,人参基质组90%醇沉组分PGP-90还原糖含量达到0.24 mg/g;发酵至第40 d时,HEP-90蛋白质含量达到0.54 mg/g,PGP-90蛋白质含量达到0.46 mg/g。菌质组各醇沉组分在发酵至第24 d时对DPPH自由基的清除能力均达到较好的效果,发酵菌质中不同醇沉组分多糖HEP-40、HEP-70、HEP-90的DPPH自由基清除率分别为37.66%、60.06%、73.58%;当发酵至第40 d时,HEP-40、HEP-70、HEP-90对羟自由基的清除率分别为33.71%、54.32%、94.90%;当发酵至第40 d时,HEP组的ABTS自由基清除能力达到峰值,HEP-40、HEP-70、HEP-90对ABTS自由基的清除率分别为44.83%、87.90%、98.90%。结论:猴头菌与人参进行双向固体发酵后,发酵产物(HEP组)中的药效活性成分与未经发酵处理的人参(PGP组)中的药效活性成分相较抗氧化能力显著提升。Objective:To better study and make use of the medicinal value of Hericium erinaceus and ginseng.Methods:In this paper,Hericium erinaceus was used as the fermentation strain and ginseng was used as the drug substrate(PGP)for two-way solid fermentation to obtain Hericium erinaceus ginseng two-way solid fermentation bacteria(HEP).The contents of polysaccharides and proteins of fermentation bacteria and different alcohol precipitation components of ginseng drug substrate group in different fermentation periods were determined by phenol sulfuric acid method and Coomassie brilliant blue method.And DPPH·,·OH and ABTS+·methods were used to determine the antioxidant capacity of different alcohol precipitation components.Results:The total sugar content of 40%alcohol precipitation component was the most.At the 9th day of fermentation,the total sugar content of 40%alcohol precipitation component HEP-40 in fermentation substrate group reached 0.98 mg/g,and the total sugar content of 40%alcohol precipitation component PGP-40 in ginseng matrix group reached 1.03 mg/g.The content of reducing sugar and protein in 90%alcohol precipitation component was the most.At the 30th day of fermentation,the content of reducing sugar in 90%alcohol precipitation component HEP-90 of fermentation substrate group reached 0.18 mg/g,and the content of reducing sugar in 90%alcohol precipitation component PGP-90 of ginseng matrix group reached 0.24 mg/g;At the 40th day of fermentation,the protein content of HEP-90 and PGP-90 reached 0.54 and 0.46 mg/g respectively.The scavenging of DPPH radicals by all alcoholic fractions of the fermentation plasm up to the 24th day of fermentation was good,with DPPH scavenging rates of 37.66%,60.06%and 73.58%for the polysaccharides HEP-40,HEP-70 and HEP-90 in the fermentation plasm,respectively;At the 40th day of fermentation,the clearance rates of HEP-40,HEP-70 and HEP-90 on OH radical were 33.71%,54.32%and 94.90%,respectively;When the fermentation reached the 40th day,the clearance ability of the HEP group reac

关 键 词：猴头菌 人参 双向固体发酵 分级醇沉 抗氧化活性 

分 类 号：S567.51[农业科学—中草药栽培] TQ920.9[农业科学—作物学]