In-silico and in-vitro evaluation of docetaxel and berberine as potential p53 modulating apoptotic inducers in oral squamous cell carcinoma  

作　　者：Smrutipragnya Samal Rajesh Kumar Meher Debasmita Dubey Showkat Ahmad Mir Binata Nayak Mahesh Chandra Sahu Pradeep Kumar Naik Goutam Rath Santosh Kumar Swain 

机构地区：[1]Department of Otorhinolaryngology and Head and Neck Surgery,IMS and SUM Hospital,Siksha‘O’Anusandhan Deemed to be University,K8,Kalinga Nagar,Bhubaneswar-751003,Odisha,India [2]Centre of Excellence in Natural Products and Therapeutics,Department of Biotechnology and Bioinformatics,Sambalpur University,Jyotivihar,Burla,Sambalpur,Odisha,India [3]Medical Research Laboratory,IMS and SUM Hospital,Siksha‘O’Anusandhan Deemed to be University,K8,Kalinga Nagar,Bhubaneswar-751003,Odisha,India [4]School of Life Sciences,Sambalpur Univesity,JyotiviharBurla,Sambalpur,Odisha,India [5]ICMR-Regional Medical Research Centre,Chandrasekharpur,Bhubaneswar-751023,India [6]School of Pharmaceutical Sciences,Siksha‘O’Anusandhan Deemed to be University,Bhubaneswar-751030,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第12期530-540,共11页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To investigate the interaction of p53 with docetaxel and berberine and their anticancer activities against oral squamous cell carcinoma.Methods:The interaction between p53 with docetaxel and berberine was investigated and their mechanisms of action against oral squamous cell carcinoma were studied.Toxicity studies were performed to determine any toxic impact of the drugs on the vital organs of tested animals.Results:In silico results revealed the molecular interaction of docetaxel and berberine with p53 and the molecules were found to be potential p53 inducers.Docetaxel and berberine inhibited the proliferation of cancer cells in a concentration-dependent manner.Flow cytometry analysis revealed that docetaxel and berberine at IC50 concentrations upregulated the expression of p53 in oral squamous cell carcinoma cells,thus triggering apoptotic cell death.In addition,no toxicity was observed in the liver and kidney tissues of mice after docetaxel and berberine treatment.Conclusions:Docetaxel and berberine significantly suppressed the proliferation of oral cancer cells by activating p53 expression and causing apoptotic cell death.Both compounds can be potential agents for the treatment of oral cancer,with little to no toxicity at the tissue level.

关 键 词：DOCETAXEL BERBERINE PC-3 OVCAR-3 OSCC SCC-29B P53 Anticancer Apoptosis 

分 类 号：R739.8[医药卫生—肿瘤]