TFRC参与调控血管和神经元相关基因表达  

Involvement of TFRC in the regulation of vascular and neuronal related gene expression

作　　者：韩君 HAN Jun(Beijing Tcmages Pharmaceutical Co.,Ltd.,Beijing 101301,China)

机构地区：[1]北京康仁堂药业有限公司,北京101301

出　　处：《华夏医学》2022年第5期17-22,共6页Acta Medicinae Sinica

摘　　要：目的:探讨TFRC对肝癌HepG2细胞转录组水平的影响,解读其潜在的功能。方法:根据癌症基因组图谱(TCGA)数据分析,发现TFRC与肝癌患者的预后密切相关,采用TFRC敲低的肝癌HepG2细胞和阴性对照肝癌HepG2细胞进行转录组RNA测序,筛选差异表达基因并进行GO注释分析,KEGG富集分析和蛋白互作网络分析。结果:两种HepG2细胞转录组中筛选出869个差异表达基因(DEGs),功能分析发现多个DEGs与血管和神经元调节有关;依据血管和神经元相关的DEGs,构建调控网络,发现TFRC是该网络的节点基因。结论:TFRC是血管和神经元调控的重要基因。Objective: To investigate the effect of TFRC on the transcriptome level of hepatocellular carcinoma HepG2 cells and to decipher its potential function. Methods: TFRC was closely associated with the prognosis of hepatocellular carcinoma patients as determined by the Cancer Genome Atlas(TCGA) database. TFRC knockdown hepatocellular carcinoma HepG2 cells and negative control hepatocellular carcinoma cells were selected for transcriptome RNA sequencing and screening of differentially expressed genes to have GO annotation analysis, KEGG enrichment analysis and protein interaction network analysis. Results: A total of 869 differentially expressed genes(DEGs) were screened from the transcriptomes of the two HepG2 cells, and functional analysis revealed that several DEGs were associated with vascular regulation and neurons. Functional analysis revealed that several DEGs were related to vascular regulation and neurons. In addition, TFRC was found to be a node gene in the regulatory network constructed according to vascular regulation and neuron-related DEGs. Conclusion: TFRC expression is important for vascular and neuronal regulation.

关 键 词：TFRC RNA-SEQ 差异表达基因 血管调节 神经元调控 

分 类 号：R735.7[医药卫生—肿瘤]