水产品中人鱼共患细菌性病原分子生物学检测方法研究进展  

Research progress on molecular biological detection methods for bacterial pathogen common to humans and fish in aquatic products

作　　者：高子惠 朴永哲 宫月 张璜 郑秋月 曹际娟 GAO Zi-Hui;PIAO Yong-Zhe;GONG Yue;ZHANG Huang;ZHENG Qiu-Yue;CAO Ji-Juan(Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education,College of Life Science,Dalian Minzu University,Dalian 116600,China;Dalian Customs Technology Center,Dalian 116001,China;Guangzhou Double Helix Gene Technology Co.,Ltd.,Guangzhou 510630,China)

机构地区：[1]大连民族大学生命科学学院,生物技术与资源利用教育部重点实验室,大连116600 [2]大连海关技术中心,大连116001 [3]广州双螺旋基因技术有限公司,广州510630

出　　处：《食品安全质量检测学报》2022年第22期7236-7245,共10页Journal of Food Safety and Quality

基　　金：大连市揭榜挂帅项目(2021JB12SN039);辽宁省“兴辽英才计划”项目(XLYC2002106)。

摘　　要：水产行业是国民经济重要产业之一,水产养殖过程中病原微生物造成鱼类病害频发,人鱼共患病原菌会对人类健康构成威胁,水产品安全问题日益突出。人鱼共患病原菌检测方法对于病害的发现、预防及水产行业的健康可持续发展具有重要意义。人鱼共患病原菌包括人鱼共患细菌性病原和人鱼共患寄生虫病原等。本文对水产品中常见的人鱼共患细菌性病原的种类及其危害性、细菌性病原分子生物学检测方法的原理和应用及成簇的规律间隔的短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)基因编辑技术在病原菌检测方面的最新研究进展进行了综述,为研发易携带且简便快速的新型核酸检测方法及产品及水产品中人鱼共患病原菌的检测和防治提供参考和借鉴。The aquatic industry is one of the important industries in the national economy.In the process of aquaculture,pathogenic microorganisms cause frequent fish diseases,and the bacterial pathogen common to humans and fish also pose a threat to human health.The safety of aquatic products is becoming increasingly prominent.The detection methods of bacterial pathogen common to humans and fish is of great significance for the discovery and prevention of diseases and the healthy and sustainable development of the aquatic industry.The pathogenic bacteria common to humans and fish include symbiotic bacterial pathogens and symbiotic parasitic pathogens.This paper reviewed the species and harmfulness of common fish and human bacterial diseases in aquatic products,the principle and application of molecular biology detection methods for bacterial pathogen,and the latest research progress of clustered regularly interspaced short palindromic repeats(CRISPR)gene editing technology in pathogenic bacteria detection,so as to provide references for the research and development of portable,simple and rapid new nucleic acid detection methods,products and the detection and prevention of bacterial pathogen common to humans and fish in aquatic products.

关 键 词：水产品 人鱼共患细菌性病原 分子生物学 成簇的规律间隔的短回文重复序列 

分 类 号：R155.5[医药卫生—营养与食品卫生学]