一株同步糖化发酵纤维素产乙醇的尖孢镰刀菌的筛选  

Screening of Excellent Fusarium oxysporum Strains for Simultaneous Saccharification and Fermentation of Bagasse to Ethanol

作　　者：周泉 湛佳佳 李璧 张志旭[3,4] 秦丹[1] 曾璐[1] ZHOU Quan;ZHAN Jiajia;LI Bi;ZHANG Zhixu;QIN Dan;ZENG Lu(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;Medicine and Technology College,Zunyi Medical University,Zunyi 563000,China;College of Horticulture,Hunan Agricultural University,Changsha 410128,China;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]遵义医科大学医学与科技学院医养健康学院,贵州遵义563000 [3]湖南农业大学园艺学院,湖南长沙410128 [4]湖南农业大学园艺作物种质创新与新品种选育教育部工程研究中心,湖南长沙410128

出　　处：《食品安全导刊》2022年第35期149-154,共6页China Food Safety Magazine

基　　金：湖南省大学生创新创业训练计划项目(S202110537006);湖南省教育厅项目(19C0941);作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目(19KFXM08);云南特异辣椒资源高效利用研究(202202AE090031)。

摘　　要：为获得一株具有同步糖化发酵纤维素产乙醇能力的优良尖孢镰刀菌菌株,研究其产醇性能。本研究通过刚果红平板染色、滤纸崩解对10株尖孢镰刀菌进行筛选,对其产酶及五碳糖利用性能进行测定,并以甘蔗渣为底物,结合傅立叶变换红外吸收光谱仪(Fourier Transform Infrared Spectroscopy,FTIR)对底物成分变化情况进行测定,进行了同步糖化发酵性能的分析。结果表明,菌株FOC-mh2-2具有较强的降解能力,其纤维素酶系统较为完全,内切酶、外切酶、β-葡聚糖酶、木聚糖酶活力分别可达到15.32 U·mL^(-1)、23.56 U·mL^(-1)、10.65 U·mL^(-1)和249.05 U·mL^(-1),可使五碳糖的乙醇产量达16.84 g·L^(-1),以甘蔗渣作为纤维素底物材料进行同步糖化发酵,乙醇转化率可达19 g·kg^(-1),降解率达28%。经FTIR成分分析,FOC-mh2-2在发酵过程中可使木质素含量降低,木质素官能团被解聚的同时,纤维素和半纤维素的空间结构暴露,为菌株的同步糖化发酵提供了良好的底物条件。In order to screen a Fusarium oxysporum strain with the ability of simultaneous saccharification and fermentation of cellulose to ethanol,and explore its ethanol production performance.In this study,through Congo red plate dyeing,filter paper disintegration,an excellent strain FOC-mh2-2 was screened from 10 Fusarium oxysporum strains,and the properties of producing enzyme and utilizing pentose were determined.The performance of simultaneous saccharification and fermentation was analyzed by using bagasse as substrate,and measuring the change of substrate composition combined with Fourier Infrared Spectrum Determination(FTIR).The results showed that the strain FOCmh2-2 had strong degradation ability and complete cellulase system,the activities of endoglucanase,exoglucanase,β-glucanase and xylanase were 15.32 U·mL^(-1)、23.56 U·mL^(-1)、10.65 U·mL^(-1)、249.05 U·mL^(-1),and the ethanol yield of pentose was 16.84 g·L^(-1).When bagasse was used as lignocellulose substrate for simultaneous saccharification and fermentation,the ethanol conversion rate could reach 19 g·kg^(-1),and the degradation rate of bagasse could reach 28%.The results of FTIR analysis showed that,FOC-mh2-2 could reduce the content of lignin during the fermentation process.The lignin functional group was depolymerized and the spatial structure of cellulose and hemicellulose was exposed,which provided a good substrate condition for the simultaneous saccharification and fermentation of the strain.

关 键 词：尖孢镰刀菌 同步糖化发酵 甘蔗渣 乙醇 纤维素酶 

分 类 号：TQ223.122[化学工程—有机化工] TQ920.1[轻工技术与工程—发酵工程]