高压二氧化碳对人冠状病毒(hCoV-229E)的消杀效果  

Disinfection Effect of High-pressure Carbon Dioxide on Human Coronavirus(hCoV-229E)

作　　者：宁鹏 杨东 廖小军 魏凡华[1] 饶雷 毕玉海[2,4] NING Peng;YANG Dong;LIAO Xiaojun;WEI Fanhua;RAO Lei;BI Yuhai(College of Agriculture,Ningxia University,Yinchuan 750021,China;CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Center for Influenza Research and Early-warning(CASCIRE),Chinese Academy of Sciences,Beijing 100101,China;College of Food Science and Nutritional Engineering,China Agricultural University,National Engineering Research Center for Fruit&Vegetable Processing,Key Laboratory of Fruit&Vegetable Processing,Ministry of Agriculture and Rural Affairs,Beijing Key Laboratory for Food Non-thermal Processing,Beijing 100083,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]中国科学院流感研究与预警中心,中国科学院微生物研究所,病原微生物与免疫学重点实验室,北京100101 [3]中国农业大学食品科学与营养工程学院,国家果蔬加工工程技术研究中心,农业农村部果蔬加工重点实验室,食品非热加工北京市重点实验室,北京100083 [4]中国科学院大学,北京100049

出　　处：《食品工业科技》2023年第2期293-298,共6页Science and Technology of Food Industry

基　　金：北京市科技计划(Z201100009420002);中国科学院战略性先导科技专项(XDB29010102);中国科学院青年创新促进会(Y2021034);国家自然科学基金项目(32041010);国家科技资源共享服务平台(国家病原微生物资源库-NPRC-32)。

摘　　要：目的:探究高压二氧化碳(HPCD)对人冠状病毒229E(hCoV-229E)的消杀效果。方法:采用HPCD在不同温度(10、25、37℃)、压力(6.3、10 MPa)和时间(15、30 min)条件下,对hCoV-229E进行消杀处理。结果:与不加压相同温度处理的对照组相比,HPCD在10℃、6.3 MPa条件下对50 mL离心管中的病毒液处理30 min后,病毒滴度显著降低(P<0.05);在37℃、10 MPa条件下对50 mL离心管中的病毒液处理15 min后,病毒滴度极显著降低(P<0.01);在37℃、10 MPa条件下对表面含有病毒的三文鱼肉、虾壳、聚乙烯类包装材料处理15 min,病毒滴度显著降低(P<0.05)。结论:HPCD在37℃、10 MPa条件下处理15 min,可以有效消杀食品(三文鱼肉和虾壳)及聚乙烯类包装材料表面的hCoV-229E。Objective:Exploring the disinfection effect of high-pressure carbon dioxide(HPCD)on human coronavirus 229E(hCoV-229E).Methods:The human coronavirus 229E(hCoV-229E)was treated by HPCD at different temperatures(10,25,and 37℃)and pressures(6.3 and 10 MPa)for different time(15 and 30 min).Result:Compared with the control groups under the corresponding temperatures,the virus titer in 50 mL tube treated with HPCD at 10℃and 6.3 MPa for 30 min was significantly decreased(P<0.05).The virus titer was also significantly reduced after treatment with HPCD at 37℃and 10 MPa for 15 min(P<0.01).Moreover,the virus titers inoculated on the surfaces of salmon meat,shrimp shell,and polyethylene packing materials were all significantly decreased after HPCD treatment at 37℃and 10 MPa for 15 min as compared with the corresponding control groups(P<0.05).Conclusion:HPCD treatment at 37℃and 10 MPa for 15 minutes could effectively disinfect hCoV-229E on the surface of food(salmon meat and shrimp shell)and polyethylene packaging materials.

关 键 词：高压二氧化碳 人冠状病毒 食品表面 包装材料 消杀 

分 类 号：TS201.1[轻工技术与工程—食品科学]