超高效液相色谱-串联质谱法测定白酒中氨基甲酸乙酯含量的研究  

Research on Detection of Ethyl Carbamate in Baijiu by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry Detection Method

作　　者：孙贺 徐君[1] 赵颖 陈路露[1] 孟阳 SUN He;XU Jun;ZHAO Ying;CHEN Lulu;MENG Yang(Anhui Kozi Distillery Co.,Ltd.,Huaibei 235000,Anhui,China)

机构地区：[1]安徽口子酒业股份有限公司,安徽淮北235000

出　　处：《酿酒》2023年第1期105-107,共3页Liquor Making

摘　　要：对检测白酒中氨基甲酸乙酯建立一种超高效液相色谱-串联质谱的检测方法,同时采用Waters HSS T3色谱柱,以甲醇和0.1%(v/v)甲酸水溶液为流动相进行梯度洗脱,流速为0.2 m L/min;在电喷雾电离(ESI)正离子模式下,采用多重反应检测(MRM)。结果表明,该方法的标准曲线线性良好,相关系数r=0.999,检出限(3S/N)为1.0μg/L,通过加标验证,回收率范围为106%~110%,重复测定酒样6次,相对标准偏差RSD为1.57%。该方法快捷、简单、高效,可作为检验和验证技术使用。An ultra-high performance liquid chromatography-tandem mass spectrometry detection method was established for the detection of ethyl carbamate in Baijiu, and the Waters HSS T3column was used. For mobile phase, methanol and 0.1%(v/v) formic acid was used for gradient elution with a flow rate of 0.2 m L/min. Under electrospray ionization(ESI) positive ion mode, multiple reaction monitoring(MRM) is employed. The results show that the standard curve of the method has good linearity. The correlation coefficient r=0.999, the detection limit(3S/N) is 1.0 μg/L, and the recovery range is 106%~110%. The liquor samples are measured 6 times, and the relative standard deviation RSD is 1.57%. This method is quick, simple and efficient, and can be used as an inspection and verification technique.

关 键 词：氨基甲酸乙酯 白酒 液质联用仪 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程]