健康婴儿粪便样本中加氏乳杆菌的分离鉴定及安全性评价  

Isolation,Identification and Safety Evaluation of Lactobacillus gasseri from Fecal Samples of Healthy Infants

作　　者：李国花 赵馨 张明新 马雨哲 张歆怡 钱卫生 鲁曦 LI Guohua;ZHAO Xin;ZHANG Mingxin;MA Yuzhe;ZHANG Xinyi;QIAN Weisheng;LU Xi(College of Food and Bioengineering,Shaanxi University of Science and Technology,Xi'an 710021,China;Tangdu Hospital of Air Force Military Medical University,Xi'an 710032,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]空军军医大学唐都医院,陕西西安710032

出　　处：《食品工业科技》2023年第3期1-10,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金(31970115);陕西省重点研发计划(2022NY-030)。

摘　　要：目的:本研究从125例健康婴儿粪便样本中分离鉴定出加氏乳杆菌(Lactobacillus gasseri)并对其安全性和抑菌性能进行评价。方法:本研究以1月龄健康婴儿粪便样本为对象,经过MRS培养基分离纯化、革兰氏染色、生化鉴定和16S rRNA测序技术进行菌种鉴定;随后,对分离得到的加氏乳杆菌利用血琼脂平板进行溶血性评价;利用微量肉汤稀释法对常见抗生素的药物敏性进行测定,并对加氏乳杆菌的安全性进行评价;最后,采用牛津杯法检测对大肠杆菌ATCC 25922和金黄色葡萄球菌ATCC 25923的抑制作用。结果:从125例健康婴儿粪便样本中成功分离并鉴定出15株加氏乳杆菌。溶血性检测结果显示,14株均无溶血性;待测菌株对四环素、万古霉素、利奈唑胺、利福平敏感性较高,对亚胺培南完全敏感,对甲氧苄啶完全耐药;抑菌实验结果显示,有6株菌同时对大肠杆菌和金黄色葡萄球菌具有抑菌效果。结论:本研究从健康婴儿粪便样本中成功分离筛选出3株安全性较高且对大肠杆菌和金黄色葡萄球菌同时具有一定抑制作用的加氏乳杆菌,编号分别为:LGI 6-1、LGI 6-2、LGI 6-3,作为潜在的益生菌资源,为后续深入探究加氏乳杆菌功能提供研究对象。Objective:To isolate and identify Lactobacillus gasseri from 125 fecal samples of healthy infants,and to evaluate its safety and antimicrobial activity.Methods:In this study,fecal samples of 1-month-old healthy infants were taken,and the strains were separated by MRS medium,Gram staining,biochemical identification and 16S rRNA sequencing technology were used to strain identification.Subsequently,the hemolysis of the isolated L.gasseri was evaluated by blood agar.The drug sensitivity of antibiotics was determined by micro broth dilution method,and the safety of the isolated strains were evaluated.Finally,the inhibitory effects of the 15 isolated L.gasseri strains on E.coli ATCC 25922 and S.aureus ATCC 25923 were detected by Oxford cup method.Results:Fifteen strains of L.gasseri were successfully isolated from 125 fecal samples of healthy infants.Hemolysis test results showed that 14 strains of them was hemolytic negative.The 15 tested strains were highly sensitive to tetracycline,vancomycin,linezolid and rifampicin,completely sensitive to imipenem and completely resistant to trimethoprim.The results of antibacterial activity test showed that 6 strains could effectively in inhibiting both E.coli and S.aureus.Conclusion:In this study,three strains of L.gasseri were successfully isolated from healthy infant’s fecal samples,which could not only inhibit E.coli and S.aureus,but also passed the safety test(LGI 6-1,LGI 6-2 and LGI 6-3).As potential probiotic resources,they could provide a research target for further research on function exploring.

关 键 词：加氏乳杆菌 婴儿粪便 分离鉴定 安全性 抑菌性 耐药性 

分 类 号：TS201.3[轻工技术与工程—食品科学]