野生牛肝菌纯种分离及液态培养促三萜类物质合成的诱导剂优化研究  

Isolation of Pure Species of Wild Boletaceae and Optimization of Inducer for Triterpenoid Synthesis in Liquid Culture

作　　者：徐伟[1] 王植朔 王瑞琦 梁珊珊 张雪 谢红瑶 吴凡 XU Wei;WANG Zhishuo;WANG Ruiqi;LIANG Shanshan;ZHANG Xue;XIE Hongyao;WU Fan(School of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品工业科技》2023年第3期213-220,共8页Science and Technology of Food Industry

摘　　要：目的:为获得牛肝菌人工纯菌株,提高液态培养菌丝及产三萜类物质的能力。方法:以云南鲜野生牛肝菌子实体为研究对象,采用组织分离法进行母体纯菌株分离并鉴定;以金属离子Ca^(2+)、亚油酸及香菇水提物作为诱导剂,研究其对液态菌丝体中三萜类物质含量的影响。结果:经多次分离纯化获得1株纯菌种NG-3,经rDNA-ITS分子生物学鉴定,NG-3为暗褐网柄牛肝菌(Phlebopus portentosus);对NG-3菌丝体进行SEM观察,在培养8~16 d时,出现具有结实能力的锁状联合形态菌丝。对3种诱导剂进行了响应面法优化,得到的结果为Ca^(2+)浓度12 mmol/L、亚油酸量4%、香菇水提物浓度150 mg/100 mL,此时三萜含量为6.77 mg/g,比未加诱导剂三萜含量提高了76.00%。结论:3种诱导剂复合使用能够促进三萜类物质合成,提高其含量,为解决野生资源不足、实现牛肝菌功能产品产业化提供技术参数。Objective:To obtain artificial pure strain of porcini and improve the ability of liquid culture mycelium and production of triterpenoid.Methods:The fresh wild Boletaceae fruit bodies from Yunnan were used for the study,and the parent pure strain was isolated and identified by tissue isolation method;Ca^(2+),linoleic acid,and mushroom aqueous extract were used as inducing agents to study their effects on the content of triterpenoids in liquid mycelium.Results:A pure strain of NG-3 was obtained by multiple purifications and identified by rDNA-ITS molecular biology as Phlebopus portentosus;the mycelium of NG-3 was observed by SEM,and the lock-like joint morphology mycelium with the ability of fruiting appeared at 8-16 d of cultivation.Three inducers were optimized by response surface methodology.The results showed that the concentration of Ca^(2+)was 12 mmol/L,the amount of linoleic acid was 4%,and the concentration of mushroom aqueous extract was 155 mg/100 mL,at which time the triterpene content was 6.77 mg/g,which was 76%higher than the triterpene content without inducer.Conclusion:The combination of three inducers can promote the synthesis of triterpenoids and increase their content,which can provide technical parameters to solve the shortage of wild resources and realize the industrialization of functional products of Boletaceae.

关 键 词：野生牛肝菌 母体纯种 液态菌丝 三萜类物质 诱导剂 

分 类 号：TS201.3[轻工技术与工程—食品科学]