人参提取物延缓果蝇衰老的作用及机制研究  

Anti-aging Effect of Ginseng Extract and Its Mechanism on Drosophila melanogaster

作　　者：王继岚 乔巨慧 刘颖[2] 赵雨初 张思雨 王思明[2] 刘美辰[2] WANG Jilan;QIAO Juhui;LIU Ying;ZHAO Yuchu;ZHANG Siyu;WANG Siming;LIU Meichen(Jilin Provincial Center for Drug Inspection,Changchun 130062,China;Northeast Asian Research Institute of Traditional Chinese Medicine,Changchun University of Chinese Medicine,Changchun 130117,Chiina)

机构地区：[1]吉林省药品审核查验中心,吉林长春130062 [2]长春中医药大学东北亚中医药研究院,吉林长春130117

出　　处：《食品工业科技》2023年第3期406-413,共8页Science and Technology of Food Industry

基　　金：吉林省重大科技专项(20210304002YY);吉林省教育厅科学技术研究项目(JJKH20210966KJ);吉林省科技发展计划项目(YDZJ202201ZYTS683)。

摘　　要：目的:基于果蝇模式生物,探讨人参提取物对果蝇衰老的延缓作用及其机制。方法:通过高效液相色谱法分析人参提取物中人参皂苷成分组成;将野生型Canton-S果蝇随机分成对照组及人参提取物组,通过寿命实验及检测摄食量、甘油三酯水平、繁殖能力、运动能力,以及在饥饿、热应激以及氧化应激条件下果蝇的存活时间来分析人参提取物的抗衰老能力;通过聚合酶链式反应测定腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)通路及哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)通路相关基因转录水平。结果:人参提取物中含13种人参皂苷成分。与对照组相比,7.5 mg/mL人参提取物对果蝇的延寿率达9.72%,且不显著影响其摄食量、甘油三酯水平以及产卵量(P>0.05),能够显著提高果蝇的攀爬能力(P<0.05或P<0.01),可显著增加果蝇抵抗压力应激能力(P<0.05或P<0.01)。与对照组相比,人参提取物的摄入显著提高了AMPKα、FoxO、Sirt1、LKB1和CaMKII的转录水平(P<0.05或P<0.01),显著降低HDAC4和SREBP的转录水平(P<0.05)、提高ATP含量和ATP/ADP比值(P<0.05或P<0.01);显著降低mTOR通路相关基因PI3K、TORC、4E-BP和S6K的转录水平(P<0.05或P<0.01),极显著提高Atg1、Atg5、Atg8a和Atg8b的转录水平(P<0.01)。结论:人参提取物可通过激活AMPK通路并抑制mTOR通路延缓果蝇衰老。Objective:Based on the model organism of Drosophila melanogaster,the anti-aging effect of ginseng extract and its mechanism on Drosophila melanogaster were investigated.Methods:The composition of ginsenosides in ginseng extract were analyzed by HPLC.Wild Canton-S Drosophila melanogaster were randomly divided into control group and ginseng extract group.The life span test,food intake,triglyceride level,reproductive ability,exercise ability,and the survival time of Drosophila melanogaster under starvation,heat stress and oxidative stress were applied to analyze the anti-aging function of ginseng extract.The transcription levels of genes related to AMP-activated protein kinase(AMPK)pathway and mammalian target of rapamycin(mTOR)pathway were determined by polymerase chain reaction.Results:Ginseng extract contained 13 ginsenosides.Compared with the control group,7.5 mg/mL ginseng extract could prolong the life span of Drosophila melanogaster by 9.72%,and had no significant effect on the food intake,triglyceride level and fecundity(P>0.05),significantly improved the climbing ability(P<0.05 or P<0.01),and increased the ability to resist stress(P<0.05 or P<0.01).Compared with the control group,the intake of ginseng extract significantly increased the transcript levels of AMPKα,FoxO,Sirt1,LKB1 and CaMKII(P<0.05 or P<0.01),decreased the transcript levels of HDAC4 and SREBP(P<0.05),and enhanced ATP content and ATP/ADP ratio(P<0.05 or P<0.01).In addition,ginseng extract significantly decreased the transcription levels of mTOR-related genes PI3K,TORC,4E-BP and S6K(P<0.05 or P<0.01),and significantly increased the transcription levels of Atg1,Atg5,Atg8a and Atg8b(P<0.01).Conclusion:Ginseng extract could delay the aging of Drosophila melanogaster by activating AMPK pathway and inhibiting mTOR pathway.

关 键 词：人参提取物 果蝇 延缓衰老 AMPK MTOR 

分 类 号：TS201.4[轻工技术与工程—食品科学]