三文鱼多肽对鱼糜制品品质的影响及其生物活性的研究  

Study on the effect of salmon polypeptide on the quality of surimi products and its biological activity

作　　者：朱芷萱 刘波 韩玲钰 蒙月月 韩世英 李金颖 崔美琪 许喆 李婷婷[1,2] ZHU Zhi-Xuan;LIU Bo;HAN Ling-Yu;MENG Yue-Yue;HAN Shi-Ying;LI Jin-Ying;CUI Mei-Qi;XU Zhe;LI Ting-Ting(College of Life Science,Dalian Minzu University,Dalian 116600,China;Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education,Dalian 116600,China;Dalian Rich Enterprise Group Co.,Ltd.,Dalian 116039,China)

机构地区：[1]大连民族大学生命科学学院,大连116600 [2]生物技术与资源利用教育部重点实验室,大连116600 [3]大连瑞驰企业集团有限公司,大连116039

出　　处：《食品安全质量检测学报》2022年第24期7833-7841,共9页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2018YFD0400601);浙江省深蓝渔业资源高效开发利用重点实验室开放基金项目(SL2021004)。

摘　　要：目的利用三文鱼加工废弃物制备多肽,探究其对鱼糜品质的影响及其生物活性。方法以三文鱼头部、鱼骨以及其他副产物废弃料为原料,通过胃蛋白酶、胰蛋白酶、中性蛋白酶进行酶解制备多肽,讨论并比较其对鱼糜品质的影响,并从体外抗氧化活性以及促骨生成活性两个角度对其生物活性进行初步探究。结果三文鱼不同部位以及不同的酶所制备的多肽对鱼糜凝胶均具有改良作用,其中,鱼骨中性酶肽效果最显著,提升了凝胶强度、持水性以及白度等指标,降低蒸煮损失以及氧化变色,且具备较好的体外抗氧化活性,同时能够促进成骨细胞的增殖分化,有效提高细胞碱性磷酸酶活性。结论三文鱼多肽对鱼糜品质具有改良作用,并具有良好的抗氧化活性及成骨活性,可为潜在抗骨质疏松活性肽的进一步开发利用提供理论基础。Objective To prepare peptides from salmon processing waste and investigate its effect on the quality of surimi and its biological activity.Methods The peptides were prepared by enzymatic digestion of salmon head,fish bone and other by-product waste materials with pepsin,trypsin and neutral protease,and their effects on the quality of surimi were discussed and compared,and their biological activities were initially investigated in terms of in vitro antioxidant activity and osteogenic activity.Results The peptides prepared from different parts of salmon and different enzymes could improve the gel of surimi,among which,the neutral enzyme peptide of fish bone had the most significant effect,which improved the gel strength,water-holding capacity and whiteness,reduced cooking loss and oxidative discoloration,and had good in vitro antioxidant activity,as well as could promote the proliferation and differentiation of osteoblasts and effectively increase the cellular alkaline phosphatase activity.Conclusion The salmon polypeptide can improve the quality of surimi and has good antioxidant and osteogenic activities,which can provide a theoretical basis for the further development and utilization of the potential anti-osteoporosis active peptide.

关 键 词：三文鱼多肽 鱼糜凝胶 鱼糜制品品质 抗氧化活性 成骨活性 碱性磷酸酶活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]