酸性电解水对低温条件下单增李斯特菌杀灭效果  

Inactivation effect of acidic electrolyzed water on Listeria monocytogenes under low temperature conditions

作　　者：梁凡 杜明[2] 潘迎捷[1,3,4] 刘海泉 赵勇[1,3,4] LIANG Fan;DU Ming;PAN Yingjie;LIU Haiquann;ZHAO Yong(College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,Liaoning,China;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306,China;Laboratory of Quality&Safety Risk Assessment for Aquatic Product on Storage and Preservation,Ministry of Agriculture and Rural Affairs,Shanghai 201306,China;Engineering Research Center of Food Thermal-processing Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [3]上海水产品加工贮藏工程技术研究中心,上海201306 [4]农业农村部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [5]上海海洋大学食品热加工工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2022年第6期1570-1581,共12页Journal of Shanghai Ocean University

基　　金：国家重点研发计划(2018YFC1602205);上海市科学技术委员会上海市“科技创新行动计划”农业领域项目(19391901600);上海市教育委员会科研创新计划(2017-01-07-00-10-E00056)。

摘　　要：单增李斯特菌是能在低温下生长的一种微生物。冷藏即食食品和冷冻食品加工设备中单增李斯特菌的生长是主要的食品安全问题。酸性电解水(AEW)是一种新型高效、安全且无残留的非热灭菌技术。AEW用于灭活4℃条件下的浮游态、生物膜态及冷藏金针菇样品上单增李斯特菌。通过检测AEW处理前后菌落总数变化、生物被膜、生物被膜结构参数以及毒力基因表达,进一步分析样品实验等。结果表明,当电解质NaCl浓度为0.10%,处理时间为1 min、30 min时,4℃和37℃下的单增李斯特菌菌落总数、生物被膜清除率存在极显著差异,相同处理时间,AEW较难杀灭4℃条件下的单增李斯特菌及难清除其生物被膜,本研究为评估冷链中食品和冷藏食品及食品加工设备的安全性提供了新的理论基础,对冷藏食品使用抗菌剂提出更高要求。Listeria monocytogenes is a psychrophilic bacterium that can grow at low temperatures. The growth of L. monocytogenes in refrigerated ready-to-eat food and frozen food processing equipment is a major food safety issue. Acidic electrolyzed water(AEW) is a new type of high-efficiency, safe and no-residue non-heat sterilization technology. AEW is used to inactivate L. monocytogenes in planktonic state, biofilm state, and frozen Flammulina velutipes samples under low temperature conditions. By detecting changes in the total number of colonies after AEW treatment, biofilm, biofilm structural parameters, and virulence gene expression, we made further analysis of sample tests, etc.. The results showed that when the electrolyte concentration was 0.10% and the treatment time was 1 min and 30 min, the total number of Listeria monocytogenes colonies and the biofilm clearance rate at 4 ℃ and 37 ℃ were extremely different. That was, with the same treatment time, AEW was more difficult to inactivate L. monocytogenes cultured at 4 ℃. Our findings may provide a theoretical basis for evaluating the safety of food and refrigerated food and food processing equipment in the cold chain. The use of antibacterial agents is more demanding.

关 键 词：冷冻冷藏食品 低温微生物 单增李斯特菌 酸性电解水 

分 类 号：TS201.3[轻工技术与工程—食品科学]