食品中椰毒假单胞菌微滴式数字PCR定量检测方法的建立  

Establishment of Droplet Digital PCR Assay for Quantitative Detection of Pseudomonas cocovenenans in Foods

作　　者：李会杰 董莲华[2] 陈桂芳 刘思渊 杨佳怡 杨靖亚[1] LI Hui-jie;DONG Lian-hua;CHEN Gui-fang;LIU Si-yuan;YANG Jia-yi;YANG Jing-ya(College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306;Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029;College of Chemistry Engineering,Shenyang University of Chemistry Technology,Shenyang 110142)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]中国计量科学研究院前沿计量科学中心,北京100029 [3]沈阳化工大学化学工程学院,沈阳110142

出　　处：《生物技术通报》2023年第1期127-136,共10页Biotechnology Bulletin

基　　金：中国计量科学研究院基本科研业务费项目(AKYZZ2023)。

摘　　要：椰毒假单胞菌可污染包括发酵米面等多种食物,其产生的两种毒素导致严重的食物中毒,威胁人体健康。基于数字PCR技术,建立了针对椰毒假单胞菌的定量测量方法。该方法具有较好的特异性与重复性,人工污染糯米汤样品中的检出限为361 CFU/mL。与平板培养法相比,基于数字PCR技术的测量方法缩短了测量时间,有利于椰毒假单胞菌的快速准确测量,未来可在食品安全监测与中毒分析等领域进行应用。Pseudomonas cocovenenans contaminates a variety of foods including fermented rice and noodles.It produces two toxins that cause severe food poisoning,which threatens human health.Based on digital PCR technology,the quantitative method targeting to P.cocovenenans was established.It demonstrated good specificity and repeatability.The detection limit in polluted glutinous rice soup was 361 CFU/mL.Compared with plate counting method,the detection time was shortened obviously via the digital PCR quantitative method,which was conducive to the rapid and accurate measurement of P.cocovenenans.Thus,it may be applied in the fields of food safety monitoring and poisoning analysis in the future.

关 键 词：椰毒假单胞菌 食物中毒 数字PCR 平板培养法 定量测量 

分 类 号：TS207.5[轻工技术与工程—食品科学]