邻苯二甲酸二(2-乙基己基)酯的遗传毒性研究  

Genotoxicity of Di(2-ethyl hexyl) phthalate

作　　者：李军涛[1] 李文学[1] 张岩 杨光宇[1] 庄润璇 张晓君 杨辉 张波 朱伟[1] 杨颖[4] LI Juntao;LI Wenxue;ZHANG Yan;YANG Guangyu;ZHUANG Runxuan;ZHANG Xiaojun;YANG Hui;ZHANG Bo;ZHU Wei;YANG Ying(Guangzhou Center for Disease Control and Prevention,Guangdong Guangzhou 510440,China;School of Public Health,Southern Medical University,Guangdong Guangzhou 510515,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China;Guangdong Provincial Center for Disease Control and Prevention,Guangdong Guangzhou 511430,China)

机构地区：[1]广州市疾病预防控制中心,广东广州510440 [2]南方医科大学公共卫生学院,广东广州510515 [3]国家食品安全风险评估中心,北京100022 [4]广东省疾病预防控制中心,广东广州511430

出　　处：《中国食品卫生杂志》2022年第5期878-883,共6页Chinese Journal of Food Hygiene

基　　金：国家重点研发计划(2018YFC1603100);广东省重点领域研发计划(2019B020210003)。

摘　　要：目的 评价邻苯二甲酸二(2-乙基己基)酯(DEHP)的遗传毒性。方法 采用细菌回复突变试验(Ames试验)、体外细胞染色体畸变、微核和彗星试验、体内小鼠骨髓红细胞微核试验、小鼠精原细胞染色体畸变试验评价DEHP诱发基因突变、染色体畸变及DNA损伤等遗传毒性。结果 Ames试验结果为阴性;中国仓鼠肺(CHL)细胞遗传毒性测试结果显示,DEHP各剂量组(0.312 5~5 mg/mL)染色体畸变率与溶剂对照组差异不具有统计学显著性(P>0.05),高剂量(2.5和5.0 mg/mL)下微核率升高,5.0 mg/mL剂量下彗星尾部DNA含量增加,与溶剂对照组相比差异具有统计学显著性(P<0.05)。DEHP各剂量组(3.75~15 g/kg)小鼠骨髓红细胞微核率与阴性对照组差异不具有统计学显著性(P>0.05)。小鼠精原细胞染色体畸变结果显示,阴性对照组、3.75(24 h)、7.5(24 h)、15(24 h)及15 g/kg·BW(48 h)精原细胞染色体畸变率分别为1.6%、1.4%、1.8%、2.4%和3.8%,虽有增加的趋势,组间差异不具有统计学显著性(P>0.05)。结论 本试验条件下未观察到DEHP诱发基因突变、染色体畸变和DNA损伤。Objective To assess the genotoxicity of Di(2-ethylhexyl) phthalate(DEHP).Methods A series of guideline-compliant assays were performed to evaluate mutagenicity,chromosomal or DNA damage of DEHP,including Ames test,in vitro mammalian chromosome aberration assay and micronucleus test,comet assay,in vivo mice bone marrow micronucleus assay,and spermatogonial chromosomal aberration test.Results Ames tests results were negative. In in vitro tests,DEHP did not increase the frequency of chromosomal aberration,micronucleus and DNA break except in the dose of 2. 5 and 5. 0 mg/mL,which significantly increased the frequency of micronucleus and DNA break. In Vitro mammalian cell micronucleus test was negative. The chromosomal aberration rates in mice spermatogonial cells were 1. 6%,1. 4%,1. 8%,2. 4% and 3. 8% in negative control,3. 75(24 h),7. 5(24 h),15(24 h) and 15(48 h) mg/kg·BW of DEHP,respectively.There was an increasing trend without statistical significant between groups.Conclusion DEHP showed no obvious genotoxicity under the test conditions.

关 键 词：邻苯二甲酸二(2-乙基己基)酯 基因突变 DNA损伤 染色体畸变 

分 类 号：R155[医药卫生—营养与食品卫生学]