贵州传统小曲酵母菌分子指纹图谱分析  

Molecular Fingerprinting Analysis of Yeasts Isolated from Traditional Guizhou Xiaoqu,a Traditional Fermentation Starter

作　　者：王春晓 何宇淋 唐佳代 邱树毅 WANG Chunxiao;HE Yulin;TANG Jiadai;QIU Shuyi(Province Key Laboratory of Fermentation Engineering and Biopharmacy,School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Department of Liquor Engineering,Moutai Institute,Renhuai 564500,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]茅台学院酿酒工程系,贵州仁怀564500

出　　处：《食品科学》2023年第2期165-172,共8页Food Science

基　　金：国家自然科学基金青年科学基金项目(31801523,32160544,32060518);贵州省科技计划项目(黔科合支撑[2022]重点006号);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]120);贵州省发酵工程与白酒酿造人才基地项目(黔人领发[2018]3号);贵州大学引进人才科研项目(贵大人基合字[2017]44号,贵大培育[2020]50号);遵义市创新人才团队培养项目(遵义科人才[2020]9)。

摘　　要：采用26S rRNA基因D1/D2区域序列分析方法将59株分离自贵州5个不同地区传统小曲的酵母菌鉴定为6个酵母菌种:1株阿萨丝孢酵母(Trichosporon asahii)、32株扣囊覆膜孢酵母(Saccharomycopsis fibuligera)、8株Saccharomycopsis malanga、4株伯顿丝孢毕赤酵母(Hyphopichia burtonii)、6株异常威克汉姆酵母(Wickerhamomyces anomalus)和8株酿酒酵母(Saccharomyces cerevisiae)。本研究展示了6个酵母菌种的WL培养基菌落特征和5.8S rRNA基因ITS区限制性片段长度多态性酶切图谱特征,为这6个菌种的快速鉴定提供依据。在菌种鉴定的基础上,本研究进一步采用串联重复-tRNA指纹图谱法分析酵母菌的种内差异,共展示了17个基因型,H.burtonii、W.anomalus与S.fibuligera分别鉴定出4、3种和8种基因型,其中S.fibuligera基因型10、11、12、15、16与17之间的遗传关系较近,而其余菌种内部基因型之间的遗传关系相对较远。A total of 59 yeast isolates from Xiaoqu from five different regions of Guizhou were identified to belong to six species by sequencing of the D1/D2 domain of the 26S rRNA gene,including Trichosporon asahii(one strain),Saccharomycopsis fibuligera(32 strains),Saccharomycopsis malanga(8 strains),Hyphopichia burtonii(4 strains),Wickerhamomyces anomalus(6 strains),and Saccharomyces cerevisiae(8 strains).The colony characteristics of the six yeast species on WL medium and the restriction fragment length polymorphism patterns of the internal transcribed spacer(ITS)region of the 5.8S rRNA gene of these species were explored in this study in order to provide a basis for their fast identification.Tandem repeat-tRNA(TRtRNA)fingerprinting analysis was applied to discriminate intraspecific diversity,and 17 genotypes were found.Four,three,and eight different genotypes were identified from H.burtonii,W.anomalus,and S.fibuligera,respectively.The genotypes 10,11,12,15,16 and 17 of S.fibuligera had a close genetic relation,and the other genotypes from H.burtonii,W.anomalus,and S.fibuligera showed a relative far genetic relation at the intraspecific level.

关 键 词：酵母菌 5.8S rRNA基因ITS区限制性片段长度多态性 串联重复-tRNA指纹图谱法 基因型 聚类分析 

分 类 号：TS261.1[轻工技术与工程—发酵工程]