Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos  

作　　者：Bing-Qiang He Ai-Cheng Li Yu-Xuan Hou Hui Li Xing-Yuan Zhang Hui-Fei Hao Hong-Hua Song Ri-Xin Cai Ying-Jie Wang Yue Zhou Yong-Jun Wang 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,Medical School of Nantong University,Nantong,Jiangsu Province,China [2]Department of Rehabilitation Medicine,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2023年第9期2011-2018,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31871211 (to YJunW);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)。

摘　　要：The low intrinsic growth capacity of neurons and an injury-induced inhibitory milieu are major contributo rs to the failure of sensory and motor functional recovery following spinal cord injury.Heat shock transcription factor 1(HSF1),a master regulator of the heat shock response,plays neurogenetic and neuroprotective roles in the damaged or diseased central nervous system.However,the underlying mechanism has not been fully elucidated.In the present study,we used a gecko model of spontaneous nerve regeneration to investigate the potential roles of gecko HSF1(gHSF1) in the regulation of neurite outgrowth and inflammatory inhibition of macrophages following spinal cord injury.gHSF1 expression in neurons and microglia at the lesion site increased dramatically immediately after tail amputation.gHSF1 ove rexpression in gecko primary neuro ns significantly promoted axonal growth by suppressing the expression of suppressor of cytokine signaling-3,and fa cilitated neuro nal survival via activation of the mitogen-activated extracellular signal-regulated kinase/extracellular regulated protein kinases and phosphatidylinositol 3-kinase/protein kinase B pathways.Furthermore,gHSF1 efficiently inhibited the macrophagemediated inflammatory response by inactivating 1kappa B-alpha/NF-kappaB signaling.Our findings show that HSF1 plays dual roles in promoting axonal regrowth and inhibiting leukocyte inflammation,and provide new avenues of investigation for promoting spinal co rd injury repair in mammals.

关 键 词：apoptosis GECKO heat shock factor 1 INFLAMMATION NEURON regeneration spinal cord suppressor of cytokine signaling-3 

分 类 号：R651.2[医药卫生—外科学]