CHARACTERIZING FLUORESCENCE LIFETIME OF NAD(P)H IN HUMAN LEUKEMIC MYELOID CELLS AND MONONUCLEAR CELLS  

作　　者：LI-SHENG LIN LI-NA LIU HUI-FANG HUANG YUAN-ZHONG CHEN BU-HONG LI ZHENG HUANG 

机构地区：[1]Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal University,Fuzhou 350007,P.R.China [2]Fujian Institute of Hematology,Union Hospital Fujian Medical University,Fuzhou 350001,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2013年第4期56-62,共7页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(61275216);the Fujian Provincial Natural Science Foundation(2011J06022);the Science Research foundation of Ministry of Health&United Fujian Provincial Health and Education Project for Tackling the Key Research(WKJ2008-2-049);the Program for Changjiang Scholars and Innovative Research Team in University(IRT1115).

摘　　要：The aim of this er vito study was to explore the potential of using the fluorescence lifetime of intraellular reduced nicotinamide adenine dinucleotide(phosphate)(NAD(P)H)as a label-free indicator to characterize the di ferencs between human leukemic myeloid cells and normal mononuclear cells(MNC).The steady-state and time-resolved autofuorescence of two human leukemic myeloid cell lines(K562,HL60)and MNC were measured by a spectrofuorimeter.According to excitation-enmission matrix(EEM)analysis,the optimal emission of NAD(P)H in these cells suspensions occurred at 445 nm.Furthermore,the fuorescence lifetimes of NAD(P)H in leukemic myeloid cells and MNC were determined by fitting the time-resolved autofuorescence data.The mean fuorescence lifetimes of NAD(P)H in K562,HL60,and MNC cells were 557±1.19,4.45±0.71,and 7.31±0.60 ns,respectively.There was a significant diference in the mean lifetime of NAD(P)H between leukemic myeloid cells and MNC(p<0.05).The difference was essentally caused by the change in relative concentration of free and protein-bound NAD(P)H.This study suggests that the mean fuorescence lifetime of NAD(P)H might be a potential label-free indicator for differentiating leukemic myeloid cells from MNC.

关 键 词：Leukemic myeloid cells normal mononucear cells AUTOFLUORESCENCE nicotinarmide adenine dimucleotide lifet ime DIFFERENTIATION 

分 类 号：R73[医药卫生—肿瘤]