基于转座突变的遗传学实验设计与探讨  

Design and exploration of a genetic experiment based on transposable mutation

作　　者：李楠[1] 李云峰[3] 郭海滨[1] 李亚娟[1] 许可[1] 刘自强 LI Nan;LI Yunfeng;GUO Haibin;LI Yajuan;XU Ke;LIU Ziqiang(Experimental Basis and Practical Training Center South China Agricultural University,Guangzhou 510642 China;College of Agriculture South China Agricultural University,Guangzhou 510642 China;College of Agronomy and Biotechnology Southwest University,400716 China)

机构地区：[1]华南农业大学基础实验与实践训练中心,广州510642 [2]华南农业大学农学院,广州510642 [3]西南大学农学与生物科技学院,重庆400716

出　　处：《生物学杂志》2023年第1期123-126,共4页Journal of Biology

基　　金：广东省自然科学基金项目(2021A1515010446和2019A1515012077);重庆市杰出青年基金项目(No.cstc2020jcyjjqX0020);广东省本科高校教学质量与教学改革工程建设项目(粤教高函【2021】29号);华南农业大学教学改革与研究重点项目(JG20046)。

摘　　要：转座插入突变体mf1表现为一个小穗内着生两朵以上小花。以该突变材料为切入点,将水稻幼穗分化发育、小穗形态结构观察、遗传分析和PCR检测等实验内容相结合,设计一个遗传学综合性实验。通过融合线上虚拟仿真实验与线下实操学习,学生能更加直观了解水稻的幼穗分化发育过程,理解转座因子的概念和应用、理清野生型与突变体、表型与基因型的关系、学会植物显微制片技术、PCR检测技术和方差分析方法,对培养学生多专业知识综合运用能力和科学研究能力有着重要实践意义。Transposable insertion mutant mf1 shows more than two florets in one spikelet. Taking this mutant material as the starting point, a comprehensive genetic experiment was designed by combining the experimental contents of observation of rice young panicle differentiation and development, examination of spikelet morphological structure, genetic analysis and PCR detection. Through the integration of online virtual simulation experiment and offline practical learning, students can more intuitively understand the process of rice young panicle differentiation and development, comprehend the concept and application of transposable factors, clarify the relationship between wild type and mutant and between phenotype and genotype, and learn techniques and methods of plant microsection, PCR detection and variance analysis. The implementation of the experiment has important practical significance for cultivating students’ comprehensive application ability of multi-disciplinary knowledge and scientific research ability.

关 键 词：实验教学 综合性实验 遗传学 科学研究 虚拟仿真实验 转座因子 

分 类 号：G642.423[文化科学—高等教育学] Q3-3[文化科学—教育学]