纳豆激酶微生物生产研究进展  

Research Progress of Nattokinase Microbial Production

作　　者：高梦迪 苏钱琙 李杰 樊学晶 王朝阳 邓立高[1] 李坚斌[1] GAO Meng-di;SU Qian-yu;LI Jie;FAN Xue-jing;WANG Zhao-yang;DENG Li-gao;LI Jian-bin(College of Light Industry and Food Engineering,Guangxi University,Nanning 530000,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530000

出　　处：《大豆科学》2022年第6期740-746,共7页Soybean Science

基　　金：国家自然科学基金(20864001,31160326);广西研究生教育创新计划项目(YCBZ2020018);广西自然科学基金(2022GXNSFAA035578)。

摘　　要：纳豆激酶是一种纤维蛋白降解酶,最初从纳豆芽孢杆菌发酵的蒸煮大豆中分离提取。与现有的纤溶酶相比,纳豆激酶因显示出溶纤活性强、效率高和无副作用的优点而备受关注,但其用量大、产量低、成本高的特点阻碍了其广泛应用,提高纳豆激酶产量和活性一直是推进纳豆激酶发展的重点。本文介绍纳豆激酶高产菌株的来源、常用筛选方法和高产菌株诱变方法,综述了微生物发酵纳豆激酶发酵条件优化、低成本培养原料替代及基因工程等相关研究进展,旨在为高产纳豆激酶的研究提供思路,为纳豆激酶大规模推广应用奠定基础。Nattokinase is a fibrin degrading enzyme, which was originally isolated and extracted from boiled soybeans fermented by Bacillus natto. Compared with the existing fibrinolytic enzymes, nattokinase has attracted much attention because of its strong fibrinolytic activity, high efficiency and no side effects. However, the wide application is hindered by the characteristics of large demand, low output and high cost of nattokinase. Improving the production and activity of nattokinase has always been the focus of promoting the development of nattokinase. This paper introduced the sources, commonly used screening methods and high-yield strain mutagenesis methods of nattokinase, and reviewed the research progress of microbial fermentation nattokinase fermentation conditions optimization, low-cost culture raw material substitution and genetic engineering, aiming to provide ideas for the research of high-yield nattokinase and lay the foundation for the large-scale promotion and application of nattokinase.

关 键 词：纳豆激酶 菌株筛选 菌株诱变 发酵优化 生产原料 基因工程 

分 类 号：O629.8[理学—有机化学]