基于酶催化合成单甘油酯工艺优化及抑菌活性研究  

Process optimization of enzyme-catalyzed synthesis of monoglyceride and its antibacterial activity

作　　者：李晓敏[1] Xiaomin Li(Hangzhou Vocational&Technical College,Hangzhou,Zhejiang 310018,China)

机构地区：[1]杭州职业技术学院,浙江杭州310018

出　　处：《日用化学工业（中英文）》2023年第2期180-186,共7页China Surfactant Detergent & Cosmetics

基　　金：2018年全国教育信息技术研究课题(186130051)。

摘　　要：以月桂酸和甘油为原料,叔丁醇为介质,采用脂肪酶催化合成了月桂酸单甘油酯(GML),采用单因素试验验证了GML的较佳合成工艺,并研究了GML对大肠杆菌的抑制效果,结果表明,叔丁醇与甘油的质量比为1.5∶1,脂肪酶用量为12%,反应温度70℃,甘油和月桂酸的摩尔比为3∶1,反应时间为1.5 h为较优条件。抑菌测试表明GML对大肠杆菌有着显著的抑制作用,抑菌效果随GML质量浓度增大而增强,GML质量浓度达到16 mg/mL时抑菌圈直径不再增大,在pH=5.7的弱酸环境下的抑菌效果和抑菌率的半衰期均强于pH=7.2的中性条件,72 h时pH=5.7的环境下GML对大肠杆菌的抑菌率依旧能够保持在3.9%。Lauric acid monoglyceride(GML) was synthesized,in which lauric acid and glycerol were used as raw materials,tert-butanol was used as the medium,and lipase was used to catalyze the reaction.Single-factor experiment was used to optimize the synthesis process of GML.The optimized conditions were as follows:The mass ratio of tertbutanol to glycerol was 1.5∶1,the amount of lipase was 12%,the reaction temperature was 70 ℃,the molar ratio of glycerol to lauric acid was 3∶1,and the reaction time was 1.5 h.The antibacterial test showed that GML had significant inhibitory effect against Escherichia coli,and the antibacterial effect was increased with the increase of mass concentration.The diameter of inhibition zone would not be further increased when the mass concentration of GML reached 16 mg/mL.The antibacterial effect and the half-life of bacteriostatic rate in the faintly acidic environment of pH 5.7 were both stronger than that in the neutral condition of pH 7.2,and the bacteriostatic rate of GML against Escherichia coli was still maintained at 3.9% in the environment of pH 5.7 at 72 h.

关 键 词：酶催化 脂肪酶 月桂酸单甘油酯 合成工艺 抑菌效果 

分 类 号：TQ658[化学工程—精细化工]