车前子壳诱导木聚糖酶与阿拉伯糖苷酶协同制备  

Synergistic preparation of xylanase and arabinosidase induced by psyllium husk

作　　者：杨磊 许伟 颜子怡 郑清 帅晨宇 邵荣 吴梦霖 施晓龙 YANG Lei;XU Wei;YAN Ziyi;ZHENG Qing;SHUAI Chenyu;SHAO Rong;WU Menglin;SHI Xiaolong(College of Marine and Bioengineering,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]盐城工学院海洋与生物工程学院,江苏盐城224051

出　　处：《中国酿造》2023年第1期92-97,共6页China Brewing

基　　金：国家自然科学基金青年基金项目(32001278);盐城工学院校级科研项目资助项目(154203650)。

摘　　要：该研究以车前子壳为诱导底物,采用单因素试验和响应面试验对里氏木霉(Trichoderma reesei)诱导木聚糖酶和阿拉伯糖苷酶的协同制备条件进行优化。单因素试验结果表明,在诱导碳源为车前子壳,初始pH为5.0、底物质量浓度为20 g/L、培养时间为4.0 d、碳氮比为1.6∶1.0时,木聚糖酶和阿拉伯糖苷酶活力最高,分别为(10.59±0.33)IU/mL和(8.99±0.05)IU/mL。响应面试验结果表明,最佳产酶条件为培养时间4.0 d、底物质量浓度20 g/L、碳氮比1.6∶1.0。在此最佳条件下,木聚糖酶和阿拉伯糖苷酶活力分别为(11.42±0.15)IU/mL和(10.83±0.08)IU/mL,分别是未优化前的2.55倍和7.37倍。Using psyllium husk as induction substrate,the fermentation conditions for the synergistic preparation of xylanase and arabinosidase induced by Trichoderma reesei were optimized by single-factor tests and response surface tests.The results of single-factor tests showed that the activities of xylanase and arabinosidase were the highest under the condition of psyllium husk as induced carbon source,initial pH 5.0,substrate mass concentration 20 g/L,culture time 4.0 d,and carbon and nitrogen ratio 1.6∶1.0,which were(10.59±0.33)IU/ml and(8.99±0.05)IU/ml,respectively.The response surface tests results showed that the optimized enzyme production were as follows:culture time 4.0 d,substrate mass concentration 20 g/L,and carbon and nitrogen ratio 1.6∶1.0.Under these optimal conditions,the activities of xylanase and arabinosidase were(11.42±0.15)IU/ml and(10.83±0.08)IU/ml,which were 2.55 times and 7.37 times higher than those before optimization,respectively.

关 键 词：木聚糖酶 阿拉伯糖苷酶 车前子壳 协同制备 产酶条件优化 

分 类 号：R284.2[医药卫生—中药学]