响应面法优化纳豆芽孢杆菌产纳豆激酶培养条件  

Optimization of culture conditions of Bacillus natto producing nattokinase by response surface methodology

作　　者：潘冬梅 部丽群 杨传伦 田杰伟 张心青 郭南南 冉新新 王红霞 马春峰 牟文杰 于帅帅 PAN Dongmei;BU Liqun;YANG Chuanlun;TIAN Jiewei;ZHANG Xinqing;GUO Nannan;RAN Xinxin;WANG Hongxia;MA Chunfeng;MU Wenjie;YU Shuaishuai(Chambroad Chemical Industry Research Institute Co.,Ltd.,Binzhou 256500,China;Shandong Jingbo Holding Co.,Ltd.,Binzhou 256500,China;Jingbo Agrochemical Technology Co.,Ltd.,Binzhou 256500,China)

机构地区：[1]黄河三角洲京博化工研究院有限公司,山东滨州256500 [2]山东京博控股集团有限公司,山东滨州256500 [3]京博农化科技有限公司,山东滨州256500

出　　处：《中国酿造》2023年第1期181-185,共5页China Brewing

基　　金：山东省重点研发计划(2018YYSP018)。

摘　　要：采用单因素试验及响应面试验对纳豆芽孢杆菌(Bacillus natto)培养条件进行优化。在单因素试验的基础上,取影响纳豆激酶活力的5个重要条件因子(培养温度、培养时间、初始pH、接种量、菌龄)设计5因素3水平的响应面试验,建立以纳豆激酶活力为响应值的多元二次回归方程。结果表明,纳豆芽孢杆菌最佳培养条件为:培养温度34℃、培养基初始pH值为7.5、接种量3%、培养时间95 h、菌龄17.5 h。在此最佳条件下,纳豆激酶活力达到5087 FU/mL,较优化前提高了18.83%。The culture conditions of Bacillus natto were optimized by single factor tests and response surface tests.Based on single factor tests,5 important condition factors(culture temperature,time,initial pH,inoculum,cell age)that affecting the nattokinase activity were selected to design the response surface test with 5 factors and 3 levels,and the multiple quadratic regression equation with the activity of nattokinase as the response value was established.The results showed that the optimal culture conditions of B.natto were as follows:culture temperature 34℃,medium initial pH 7.5,inoculum 3%,culture time 95 h,cell age 17.5 h.Under the optimum conditions,the nattokinase activity reached 5087 FU/ml,which was 18.83%higher than that before optimization.

关 键 词：纳豆芽孢杆菌 纳豆激酶 响应面法 优化 培养条件 

分 类 号：Q815[生物学—生物工程]