花生红衣原花青素的提取工艺及抗氧化活性研究  

Study on Proanthocyanidin Extraction Technology from Peanut Seed Coat and Its Antioxidant Activity

作　　者：沈文凤 王明清[1] 赵传志[2] 于丽娜[1] 毕洁[1] 宋昱 江晨[1] 孙杰[3] 迟晓元[1] Shen Wenfeng;Wang Mingqing;Zhao Chuanzhi;Yu Lina;Bi Jie;Song Yu;Jiang Chen;Sun Jie;Chi Xiaoyuan(Shandong Peanut Research Institute,Qingdao 266100,China;Institute of Crop Germplasm Resources,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Qingdao University,Qingdao 266071,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]山东省农业科学院农作物种质资源研究所,山东济南250100 [3]青岛大学,山东青岛266071

出　　处：《山东农业科学》2023年第1期144-149,共6页Shandong Agricultural Sciences

基　　金：青岛市科技惠民示范引导专项(20-3-4-33-nsh);山东省农业科学院农业科技创新工程项目(CXGC2022B06);山东省农业科学院揭榜科技难题项目(SHJB2021-73);山东省自然科学基金项目(ZR2021MC049);国家花生产业技术体系项目(CARS-13)。

摘　　要：本试验旨在探究花生红衣中原花青素的最佳提取条件及提取物的抗氧化活性。以花生红衣为研究对象,对8种大孔树脂通过静态吸附试验和动态解析试验进行筛选择优,然后经动态吸附试验优化其最佳吸附工艺条件,最后采用不同浓度乙醇进行洗脱提取,并对提取物进行抗氧化活性检测。结果表明:提取原花青素的较适树脂为LX-32,其最佳吸附条件为上样流速1.5 mL/min、样品浓度6.0 mg/mL。20%、40%、60%乙醇洗脱提取物中的原花青素抗氧化活性差异不大,对羟自由基、DPPH自由基和超氧阴离子自由基的清除能力分别为50%~53%、40%~44%、13%~14%。The aim of this study was to explore the optimal extraction conditions and the antioxidant activity of proanthocyanidins from peanut seed coat. Eight kinds of macroporous resin were selected by static adsorption test and dynamic analytical test. Then the optimal adsorption conditions were confirmed by dynamic adsorption test. Finally, 20%, 40% and 60% ethanol were used for elution extraction, and the antioxidant activity of the extract was detected. The results showed that LX-32 was the suitable resin for proanthocyanidin extraction, and the optimal adsorption conditions included: loading flow rate as 1.5 mL/min and sample concentration as 6.0 mg/mL. There was no significant difference in ability of the products for scavenging hydroxyl radical, DPPH radical and superoxide anion radical, and the scavenging ability to hydroxyl radical, DPPH and superoxide anion was 50%~53%, 40%~44% and 13%~14%, respectively.

关 键 词：花生红衣 原花青素 提取工艺 大孔树脂 抗氧化活性 

分 类 号：S565.209.9[农业科学—作物学]