5种食用菌水提物生物活性的比较  

Comparison of Bioactivities of Water Extracts from Five Edible Mushrooms

作　　者：韩明月 李俐 郝利民 王小龙 江庆伍 李雪 刘可可 刘永奇 伊娟娟 鲁吉珂 HAN Mingyue;LI Li;HAO Limin;WANG Xiaolong;JIANG Qingwu;LI Xue;LIU Keke;LIU Yongqi;YI Juanjuan;LU Jike(School of Life Sciences,Zhengzhou University,Zhengzhou 450001,China;The Quartermaster Equipment Institute,Academy of Military Sciences PLA China,Beijing 100010,China;Henan Yinfeng Bioengineering Technology Co.,Ltd.,Zhengzhou 450001,China;Anhui Jinzhai Qiaokang Pharmaceutical Co.,Ltd.,Lu’an 237300,China)

机构地区：[1]郑州大学生命科学学院,河南郑州450001 [2]中国人民解放军军事科学院系统工程研究院,北京100010 [3]河南省银丰生物工程技术有限公司,河南郑州450001 [4]安徽金寨乔康药业有限公司,安徽六安237300

出　　处：《食品科学》2023年第3期146-154,共9页Food Science

基　　金：国家自然科学基金青年科学基金项目(31900296);郑州大学青年人才企业合作创新团队支持计划项目(32320422);郑州大学技术开发(合作)横向项目(20210442A,20210327A);“十四五”国家重点研发计划重点专项(2022YFF1103300)。

摘　　要：目的:探究并对比草菇、灰树花、红菇、鸡腿菇、树舌5种食用菌水提物的抗氧化、免疫及辐射防护作用,为食用菌的开发利用提供理论依据。方法:采用苯酚硫酸法、考马斯亮蓝法、福林-酚法及氯化铝法分别检测5种食用菌水提物中总糖和粗多糖、总蛋白、总酚、总黄酮的质量分数;以2,2’-联氨-双(3-乙基苯并噻唑-6-磺酸)二胺盐(2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除能力、2,2-联苯基-1-苦基肼(2,2-diphenyl-1-picrylhydrazyl,DPPH)自由基清除能力、羟自由基清除能力、总还原力4种抗氧化活性指标研究食用菌水提物的体外抗氧化能力;采用细胞计数试剂盒(cell counting kit-8,CCK-8)法检测食用菌水提物对巨噬细胞RAW264.7的促增殖活性;并以60Coγ辐射氧化损伤小鼠肝细胞AML-12为模型,探究5种食用菌水提物的体外辐射防护作用。结果:草菇、灰树花、红菇、鸡腿菇、树舌5种食用菌水提物的主要成分中总糖含量占比最高,相应的总糖质量分数分别为(80.90±1.20)%、(67.00±2.48)%、(55.31±0.55)%、(84.87±7.31)%和(58.19±1.50)%。粗多糖质量分数结果显示,灰树花提取物粗多糖含量最高,其粗多糖质量分数为(61.28±2.95)%。5种食用菌水提物均具有良好的抗氧化能力,其中树舌水提物的抗氧化活性最佳。此外,不同质量浓度(10~200μg/mL)的5种食用菌水提物均能一定程度促进巨噬细胞RAW264.7的增殖,相比之下,低质量浓度树舌水提物对巨噬细胞的增殖促进作用更显著。体外辐射防护作用结果表明,在实验质量浓度范围内(10~200μg/mL),草菇水提物对60Coγ辐射所致的AML-12细胞存活率下降具有明显的改善作用,而树舌水提物在低质量浓度时也同样具有显著的辐射防护作用。结论:5种食用菌水提物具有不同的生物活性,综合比较树舌水提物的抗氧化活性、免疫调节作用和抗辐射功能均较好,�Objective:In order to provide a theoretical basis for the development and utilization of edible mushrooms,the antioxidant,immunomodulatory and anti-radiation activities of water extracts from five edible mushrooms,including Volvariella volvacea,Grifola frondosa,Russula vinosa Lindblad,Coprinus comatus and Ganoderma applanatum were studied and compared with each other.Methods:The contents of total sugar,crude polysaccharide,total protein,total phenols and total flavonoids in water extracts from the five edible mushrooms were determined by the phenol-sulfuric acid method,the Coomassie brilliant blue method,the Folin-Ciocalteu method and the aluminium chloride method,respectively.Their in vitro antioxidant activities were evaluated by 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical,2,2-diphenyl-1-picrylhydrazyl(DPPH)radical,and hydroxyl radical scavenging and total reducing power assays.Their proliferative activities on macrophage RAW264.7 cells were detected by cell counting kit-8(CCK-8)method.Their protective effects against60Coγirradiation-induced damage in mouse hepatocytes AML-12 were investigated in vitro.Results:Total sugar was the most abundant constituent in water extracts from Volvariella volvacea,Grifola frondosa,Russula vinosa Lindblad,Coprinus comatus and Ganoderma applanatum,accounting for(80.90±1.20)%,(67.00±2.48)%,(55.31±0.55)%,(84.87±7.31)%and(58.19±1.50)%of the extracts,respectively.The crude polysaccharide content of water extract from Grifola frondosa was the highest,(61.28±2.95)%.All the water extracts had good antioxidant activities,of which the water extract of Ganoderma applanatum had the best antioxidant activity.In addition,the water extracts at different concentrations(10–200μg/mL)promoted the proliferation of RAW264.7 macrophages,and the effect of the water extract of Ganoderma applanatum at low concentrations was more significant.The decreased survival rate of AML-12 cells induced by60Coγradiation could be significantly improved by the water extra

关 键 词：食用菌水提物 抗氧化活性 巨噬细胞RAW264.7 免疫调节 肝细胞AML-12 抗辐射活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]