过氧化氢和活性炭脱色对百蕊草多糖结构及应用特性影响的比较分析  

Effect of Activated Carbon or Hydrogen Peroxide Decolorization on the Structure and Application Properties of Polysaccharides from Thesium chinense Turcz

作　　者：邵丽君 琚飞龙 钟杨 周颖娣 李贝贝 凌依凡 孙玥 梁进 李雪玲 SHAO Lijun;JU Feilong;ZHONG Yang;ZHOU Yingdi;LI Beibei;LING Yifan;SUN Yue;LIANG Jin;LI Xueling(Anhui Engineering Laboratory for Agro-products Processing,School of Tea and Food Science&Technology,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学茶与食品科技学院,安徽省农产品加工工程实验室,安徽合肥230036

出　　处：《食品科学》2023年第4期146-151,共6页Food Science

基　　金：安徽省中央引导地方发展专项(20210706020015);安徽省自然科学基金项目(2108085MC122);安徽省教育厅科研项目(KJ2020A0136)。

摘　　要：采用过氧化氢氧化和活性炭吸附对百蕊草多糖进行脱色,得到HTP(过氧化氢脱色处理)和CTP(活性炭脱色处理)两种样品,采用现代仪器手段(X射线衍射仪、热重分析仪、激光粒度仪、流变仪)分析二者的物理特性,并比较CTP和HTP抑制肝癌细胞增殖活性,及其对κ-卡拉胶凝胶温度的影响。结果表明,CTP在X射线衍射图谱中表现出更加明显的晶体结构;相同添加量下,其表观黏度也高于HTP;热重曲线表明,HTP热分解程度高于CTP(HTP质量损失率59.33%,CTP质量损失率53.21%);CTP达到一定质量浓度(6~10 mg/mL)时,表现出抑制人肝癌细胞Hep G2增殖的活性,而HTP在实验质量浓度范围内(1~10 mg/m L)没有表现出抑制癌细胞增殖活性;同时,CTP对复配胶的温度滞后增加幅度大于HTP。因此,不同脱色方法会影响百蕊草多糖的结构及应用特性。Hydrogen peroxide and activated carbon were separately used for decolorization of Thesium chinense Turcz polysaccharides. The physical characteristics of the two decolorized samples were analyzed by X-ray diffractometry(XRD),thermogravimetry, laser particle size analyzer and rheometer, and their inhibitory activities against the proliferation of liver cancer cells were compared, as well as their effects on the gelation temperature of κ-carrageenan. The results showed that the crystalline structure of the activated carbon-treated sample was more obvious in the XRD pattern. Its apparent viscosity was higher than that of the hydrogen peroxide-treated sample at the same concentration. The thermogravimetric curve showed that the thermal decomposition degree of the hydrogen peroxide-treated sample was higher than that of the activated carbontreated sample(mass loss percentage of 59.33% and 53.21%, respectively). At concentrations of 6–10 mg/mL, the activated carbon-treated sample showed inhibitory activity against the proliferation of HepG2 cells, while the hydrogen peroxidetreated samples did not have any inhibitory effect in the concentration range of 1–10 mg/mL. Furthermore, the increase in temperature hysteresis of blends of the activated carbon-treated-treated polysaccharides with κ-carrageenan was greater than that in blends of the HTP-treated polysaccharides with κ-carrageenan. Therefore, different decolorization methods can affect the structure and application characteristics of Thesium chinense Turcz. polysaccharides.

关 键 词：百蕊草多糖 脱色 结构特性 卡拉胶 HEPG2 

分 类 号：TS201.7[轻工技术与工程—食品科学]