Fe^(3+)掺杂聚多巴胺纳米球荧光适配体传感器对小麦中脱氧雪腐镰刀菌烯醇的检测  

A Fluorescent Aptasensor Based on Fe^(3+) Doped Polydopamine Nanospheres for Detection of Deoxynivalenol in Wheat

作　　者：路念 马骥 成军虎[1,2,3] 孙大文 LU Nian;MA Ji;CHENG Junhu;SUN Da-wen(School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China;Academy of Contemporary Food Engineering,South China University of Technology,Guangzhou 510006,China;Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods,Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products,Guangzhou 510006,China;State Key Laboratory of Luminescent Materials and Devices,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641 [2]华南理工大学现代食品工程研究中心,广东广州510006 [3]广东省冷链食品智能感知与过程控制工程技术研究中心,广东省农产品智能冷链物流设备工程实验室,广东广州510006 [4]华南理工大学发光材料与器件国家重点实验室,广东广州510640

出　　处：《食品科学》2023年第4期313-320,共8页Food Science

基　　金：广东省基础与应用基础研究基金项目(2021A1515110396)。

摘　　要：目的:构建一种基于荧光共振能量转移(fluorescence resonance energy transfer,FRET)原理的荧光适配体传感器,用于检测小麦中的脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)。通过掺入Fe^(3+)提高传感器中聚多巴胺纳米球(polydopamine nanospheres,PDANS)对6-羧基荧光素(6-carboxyuorescein,FAM)的猝灭能力。方法:标记有FAM的DON核酸适配体(P_(DON))通过π-π堆积的非共价作用吸附到Fe^(3+)掺杂聚多巴胺纳米球(Fe-PDANS)上,由于FRET,FAM的荧光被Fe-PDANS猝灭。在DON存在的情况下,P_(DON)可以和DON特异性结合,改变P_(DON)的构象,使其难以吸附到Fe-PDANS上,从而增强传感器的荧光信号,实现对DON的检测。结果:该传感器在DON质量浓度0.266 7~133.3 ng/mL范围内具有良好的线性关系,其检出限为0.118 2 ng/mL;同时,对阳性小麦中的DON进行检测,其检测结果与酶联免疫检测法所得结果无显著差异(P>0.05)。结论:所构建的荧光适配体传感器反应快速、灵敏,且具有良好的特异性和重复性,提供了检测小麦中DON的新方法。Objective:A fluorescent aptasensor based on fluorescence resonance energy transfer (FRET) was constructed for the detection of deoxynivalenol (DON) in wheat.The ability of polydopamine nanospheres (PDANS) in the sensor to quench 6-carboxyuorescein (FAM) was improved by doping with Fe^(3+).Methods:FAM labeled DON aptamer (P_(DON)) was adsorbed on the surface of PDANS (Fe-PDANS) doped with Fe^(3+)viaπ-πstacking non-covalent interactions,and Fe-PDANS quenched the fluorescence of FAM through FRET.In the presence of DON,P_(DON)could specifically combine with DON,changing the conformation of P_(DON),making it difficult to adsorb onto Fe-PDANS,and finally enhancing the fluorescence signal of the sensor for the detection of DON.Results:The aptasensor exhibited a linear range from 0.266 7 to 133.3 ng/mL for DON,and the detection limit was 0.118 2 ng/mL.The results of this aptasensor for DON in positive wheat samples were not significantly different (P>0.05) from those obtained by enzyme-linked immunosorbent assay (ELISA).Conclusion:The fluorescent aptasensor is characterized by rapid detection,excellent sensitivity,high specificity and good repeatability,which will provide a new method for the detection of DON in wheat.

关 键 词：核酸适配体 脱氧雪腐镰刀菌烯醇 铁掺杂聚多巴胺纳米球 荧光传感器 

分 类 号：TS207[轻工技术与工程—食品科学]