scNanoATAC-seq:a long-read single-cell ATAC sequencing method to detect chromatin accessibility and genetic variants simultaneously within an individual cell  

作　　者：Yuqiong Hu Zhenhuan Jiang Kexuan Chen Zhangxian Zhou Xin Zhou Yan Wang Jingwei Yang Bo Zhang Lu Wen Fuchou Tang 

机构地区：[1]Biomedical Pioneering Innovation Center,School of Life Sciences,Peking University,Beijing,China [2]Beijing Advanced Innovation Center for Genomics(ICG),Ministry of Education Key Laboratory of Cell Proliferation and Differentiation,Beijing,China [3]PKU-TsinghuaNIBS Graduate Program,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,China [4]Peking-Tsinghua Center for Life Sciences,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,China [5]Department of General Surgery,Third Hospital,Peking University,Beijing,China

出　　处：《Cell Research》2023年第1期83-86,共4页细胞研究（英文版）

基　　金：This project was supported by the Beijing Advanced Innovation Center for Genomics and grants from the National Key R&D Program of China(2018YFA0107601).

摘　　要：Dear Editor,The accessible chromatin regions were enriched in regulatory elements in human genome and many genetic variations associated with human diseases were identified within them.1 Single-cell assay for transposase-accessible chromatin using sequencing(scATAC-seq)on the next-generation sequencing(NGS)platform is a well-established method to detect open chromatin regions within an individual cell.2 However,there remain challenges in detection of large-scale structural variations(SVs,including insertions,deletions,duplications,inversions and translocations)and haplotype phasing from scATAC-seq data,which can be well resolved by third-generation sequencing(TGS)platform-based single-molecule long-read sequencing.To integrate advantages of long-read sequencing into scATAC-seq,we developed single-cell assay for transposase‐accessible chromatin on Nanopore sequencing platform(scNanoATAC-seq),a platebased scATAC-seq method suitable for TGS platform(Supplementary information,Fig.S1).

关 键 词：METHOD INSERTION CHROMATIN 

分 类 号：Q34[生物学—遗传学]