Microvesicles with mitochondrial content are increased in patients with sepsis and associated with inflammatory responses  

作　　者：Hai-Jun Zhang Jin-Yi Li Chao Wang Guo-Qiang Zhong 

机构地区：[1]Department of Cardiology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530000,Guangxi Zhuang Autonomous Region,China [2]Department of Cardiology,The First Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421000,Hunan Province,China [3]Department of Cardiology,The Affiliated Hospital of Guilin Medical University,Guilin 541000,Guangxi Zhuang Autonomous Region,China

出　　处：《World Journal of Clinical Cases》2023年第2期342-356,共15页世界临床病例杂志

摘　　要：BACKGROUND Endothelial activation plays an important role in sepsis-mediated inflammation,but the triggering factors have not been fully elucidated.Microvesicles carrying mitochondrial content(mitoMVs)have been implicated in several diseases and shown to induce endothelial activation.AIM To explore whether mitoMVs constitute a subset of MVs isolated from plasma of patients with sepsis and contribute to endothelial activation.METHODS MVs were isolated from human plasma and characterized by confocal microscopy and flow cytometry.Proinflammatory cytokines,including interleukin(IL)-6,IL-8 and tumour necrosis factor(TNF)-α,and soluble vascular cell adhesion molecule(sVCAM)-1 were detected by ELISA.Human umbilical vein endothelial cells(HUVECs)were stimulated with the circulating MVs to evaluate their effect on endothelial activation.RESULTS MitoMVs were observed in plasma from patients with sepsis.Compared with those in healthy controls,expression of MVs,mitoMVs,proinflammatory cytokines and sVCAM-1 was increased.The number of mitoMVs was positively associated with TNF-αand sVCAM-1.In vitro,compared with MVs isolated from the plasma of healthy controls,MVs isolated from the plasma of patients with sepsis induced expression of OAS2,RSAD2,and CXCL10 in HUVECs.MitoMVs were taken up by HUVECs,and sonication of MVs significantly reduced the uptake of mitoMVs by HUVECs and expression of the above three type I IFNdependent genes.CONCLUSION MitoMVs are increased in the plasma of patients with sepsis,which induces elevated expression of type I IFN-dependent genes.This suggests that circulating mitoMVs activate the type I IFN signalling pathway in endothelial cells and lead to endothelial activation.

关 键 词：SEPSIS MICROVESICLES MITOCHONDRIA Microvesicles carrying mitochondrial content 

分 类 号：R459.7[医药卫生—急诊医学]