Huoxue Jiedu Huayu recipe(活血解毒化瘀方)alleviates contralateral renal fibrosis in unilateral ureteral obstruction rats by inhibiting the transformation of macrophages to myofibroblast  

作　　者：XIONG Yunzhao LIU Lingjin LIU Ziqian CHEN Gege HAO Juan GAO Xiaomeng QIANG Panpan WANG Zheng XU Qingyou 

机构地区：[1]Graduate School,Hebei University of Chinese Medicine,Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,China [2]Graduate School,Hebei University of Chinese Medicine Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Shijiazhuang 050091,China [3]Graduate School,Hebei University of Chinese Medicine,Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Department of Internal Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第1期105-112,共8页中医杂志（英文版）

基　　金：Supported by Natural Science Foundation-funded Project:Effect of Macrophage-to-Myofibroblast Transition in Contralateral Kidney of Unilateral Ureteral Obstruction Rats Through the Aldosterone/MR/SGK1 Pathway and Inhibition of Chinese Herbs(No.81873251);Natural Science Foundation-funded Project:Aldosterone-induced Macrophage Polarization-lymphatic Endothelial Cell-like Transformation Involved in Renal Fibrosis and the Protective Effect of Yiqi Jiedu Huayu Herbs(No.82205006);Youth Top Talent Project of Hebei Provincial Department of Education:Mechanism of Renal Fibrosis Induced by NF-kB/IL-8 Induced MMT in UUO Rats(BJK2022004)。

摘　　要：OBJECTIVE:To investigate the action and underlying mechanisms of Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)against unilateral ureteral obstruction(UUO)-induced injury in the contralateral kidney.METHODS:Forty-eight male Sprague-Dawley rats weighing(200±10)g were used in this study and randomly assigned to 4 groups:a sham group,a UUO group,a UUO+eplerenone(EPL)group,and a UUO+HJHR group.The contralateral kidneys were harvested for further study 180 d after surgery.Histological analysis,immunohistochemistry and immunofluorescence were used to study the fibrosis of the contralateral kidneys obtained from UUO rats.Contralateral kidney damagerelated pathway proteins were detected by real-time polymerase chain reaction and Western blot analysis.RESULTS:HJHR significantly inhibited fibrosis of the contralateral kidney in UUO rats by attenuating the UUOinduced macrophage-to-myofibroblast transition(MMT)in the contralateral kidney.Moreover,HJHR attenuated fibrosis in the contralateral kidney of UUO rats by preventing MMT through the aldosterone/mineralocorticoid receptor/serum/glucocorticoid regulated kinase 1 pathway.CONCLUSIONS:Our findings suggest that HJHR may be a potential treatment for renal interstitial fibrosis of obstructive nephropathy.

关 键 词：ureteral obstruction renal fibrosis ALDOSTERONE receptors mineralocorticoid MACROPHAGES MYOFIBROBLAST Huoxue Jiedu Huayu recipe 

分 类 号：R285.5[医药卫生—中药学]