发酵米荞对高脂肪秀丽隐杆线虫的降脂及抗氧化作用  

Hypolipidemic and Antioxidant Effects of Fermented Rice Buckwheat on High-fat Caenorhabditis elegans

作　　者：严静 薛秋艳 王旸 陈汶意 谢诗晴 江津津[3] 黎攀 杜冰[1] YAN Jing;XUE Qiuyan;WANG Yang;CHEN Wenyi;XIE Shiqing;JIANG Jinjin;LI Pan;DU Bing(College of Food,South China Agricultural University,Guangzhou 510642,China;Huangpu Customs Logistics Management Center,Guangzhou 510730,China;Guangzhou City Polytechnic,Guangzhou 510405,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]黄埔海关后勤管理中心,广东广州510730 [3]广州城市职业学院,广东广州510405

出　　处：《食品工业科技》2023年第6期8-15,共8页Science and Technology of Food Industry

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21);广东省重点领域研发计划项目(2020B020226008);广东省自然科学基金面上项目(2020A1515011268);广州市科技计划项目(202102080487)。

摘　　要：本文旨在研究发酵米荞的降脂及抗氧化作用。以高糖(10 mmol/L)诱导建立秀丽隐杆线虫高脂肪模型,经发酵米荞干预后,测定其对高脂肪线虫寿命、运动能力、产卵量、活性氧(ROS)水平、抵抗氧化应激能力和抗氧化酶活力的影响;同时,检测了其对高脂肪线虫体内甘油三脂和游离脂肪酸含量的影响。结果表明,经发酵米荞干预的高脂肪线虫的最大寿命极显著增加30.90%(P<0.01),运动能力极显著提升28.57%(P<0.01),产卵量增加(P>0.05);同时,发酵米荞可以极显著提高高脂肪线虫体内抗氧化酶活性(P<0.01)、降低丙二醛含量及ROS水平(P<0.01),并且极显著提升其抵抗氧化应激能力(P<0.01)。此外,发酵米荞还能极显著降低高脂肪线虫的甘油三酯及游离脂肪酸含量,分别降低了56.58%和130.54%(P<0.01)。综上所述,发酵米荞可通过增强抵抗氧化应激能力、减少脂肪沉积和提高抗氧化酶活性发挥其对高脂肪线虫的降脂作用。This research aimed to investigate the hypolipidemic and antioxidant effects of fermented rice buckwheat.A high-fat model of Caenorhabditis elegans(C.elegans)was established by high sugar(10 mmol/L)induction.After the treatment of fermented rice buckwheat,its effects on the longevity,motility,egg production,reactive oxygen species(ROS)levels,resistance to oxidative stress,and antioxidant enzyme activity of high-fat C.elegans were determined.At the same time,its effect on the triglyceride and free fatty acid content in high-fat C.elegans were examined.The results showed that the maximum life span of high-fat C.elegans intervened by fermented rice buckwheat significantly increased by 30.90%(P<0.01),significantly increased motility by 28.57%(P<0.01),and increased egg production(P>0.05).Furthermore,fermented rice buckwheat could significantly increase the antioxidant enzyme activity(P<0.01),reduce malondialdehyde content and ROS levels(P<0.01),and significantly enhance their ability to resist oxidative stress(P<0.01).In addition,fermented rice buckwheat could also significantly reduce the triglyceride and free fatty acid content of high-fat C.elegans,respectively,reduced by 56.58%and 130.54%(P<0.01).In summary,fermented rice buckwheat exhibited a lipid-lowering effect by enhancing resistance to oxidative stress,reducing fat deposition,and improving the antioxidant enzyme activity of high-fat C.elegans.

关 键 词：发酵 米荞 秀丽隐杆线虫 降脂 抗氧化活性 

分 类 号：TS213[轻工技术与工程—粮食、油脂及植物蛋白工程]