红蓝草多糖提取工艺优化及其抗氧化活性分析  

Optimization of the Extraction Process of Peristrophe roxburghiana Polysaccharide and Analysis of Its Antioxidant Activity

作　　者：陈永[1] 宋真萍 吴晖[2] 苏秀芳[1] CHEN Yong;SONG Zhenping;WU Hui;SU Xiufang(School of Chemistry and Biological Engineering,Guangxi Normal University for Nationalities,Chongzuo 532200,China;School of Food Science and Technology,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]广西民族师范学院化学与生物工程学院,广西崇左532200 [2]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《食品工业科技》2023年第5期166-173,共8页Science and Technology of Food Industry

基　　金：广西2021-2025年立项建设新增硕士学位授予单位项目(农业硕点)资助;华南理工大学国家大学科技园顺德创新园区教师科技成果转化专项资金项目(KJYS2021KZ05)。

摘　　要：为探究红蓝草多糖的最佳提取工艺及其体外抗氧化活性。试验以红蓝草为原料,探究料液比、浸提温度、浸提时间、浸提次数对红蓝草多糖得率的影响,并结合响应面法优化红蓝草多糖提取工艺,通过测定红蓝草多糖对DPPH·、·OH、ABTS^(+)·等自由基的清除能力探究其抗氧化活性。结果表明:在料液比1:31 g/mL、浸提温度85℃、浸提时间118 min、提取3次的条件下,红蓝草多糖得率最高,可达11.05%。在一定范围内,红蓝草多糖清除自由基能力与多糖的浓度呈量效关系,红蓝草多糖溶液清除DPPH·、·OH和ABTS^(+)·等自由基的IC_(50)值分别为0.18、0.73、0.64 mg/mL,说明红蓝草粗多糖具有一定的抗氧化活性。通过探究红蓝草多糖提取的最佳工艺及抗氧化活性,为今后红蓝草多糖的进一步开发与应用提供理论基础和参考。In order to explore the optimum extraction process of polysaccharide and antioxidant activity in vitro in Peristrophe roxburghiana.In this study,Peristrophe roxburghiana was used as raw material to explore the effects of solidliquid ratio,extraction temperature,time and frequency on the yield of polysaccharide.The extraction process of polysaccharide in Peristrophe roxburghiana was optimized by response surface methodology and the antioxidant activity of the polysaccharide was investigated by measuring its scavenging ability on DPPH·,·OH,ABTS^(+)·and other free radicals.The results showed that we could get the most quantity of polysaccharide extracted from Peristrophe roxburghiana,which reaches up to 11.05%,in these conditions:The solid-liquid ratio was 1:31 g/mL,the extraction temperature was 85℃,the extraction time was 118 min and the extraction frequency was 3 times.The scavenging ability of the polysaccharide against free radical was quantitatively related to the concentration of polysaccharide within a certain range.The IC_(50) values of DPPH·,·OH and ABTS^(+)·radicals scavenged by the polysaccharide solution were 0.18,0.73 and 0.64 mg/mL,respectively,indicating that the polysaccharide extracted from Peristrophe roxburghiana was with certain antioxidant activity.By exploring the optimal process and antioxidant activity of the polysaccharide extracted from Peristrophe roxburghiana,we provide a theoretical basis and reference for the further development and application of the polysaccharide in the future.

关 键 词：红蓝草 多糖 响应面优化法 抗氧化 提取工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学]