响应面法优化金钱白花蛇醇溶蛋白提取工艺  

Optimization of extraction process of gliadin from Bungarus multicinctus by response surface methodology

作　　者：刘梦 谢超 张林松 宋美营 母璇 闻崇炜[1] LIU Meng;XIE Chao;ZHANG Lin-song;SONG Mei-ying;MU Xuan;WEN Chong-wei(School of Pharmacy,Jiangsu University,Zhenjiang 212013,Jiangsu,China;Zhenjiang Food and Drug Supervision and Inspection Center,Zhenjiang 212013,Jiangsu,China)

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]镇江市食品药品监督检验中心,江苏镇江212013

出　　处：《粮食与油脂》2023年第2期73-77,共5页Cereals & Oils

基　　金：江苏省镇江市重点研发计划(社会发展)项目(SH2021010);江苏大学第20批大学生科研立项(20AD0034);江苏省大学生实践创新训练计划项目(2022102991611X)。

摘　　要：以金钱白花蛇为原料,在单因素试验基础上采用响应面法优化其醇溶蛋白提取工艺,并进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效液相色谱(HPLC)、傅里叶变换红外光谱(FTIR)指纹图谱分析。结果表明:最佳提取工艺条件为乙醇体积分数40%、料液比1∶17(g/mL)、提取时间4 h、提取温度40℃,此时醇溶蛋白得率为0.51%。SDS-PAGE指纹图谱分析表明金钱白花蛇醇溶蛋白分子质量主要介于26.69~88.02 kDa,26.69~28.34 kDa和70.84~88.02 kDa处的蛋白质丰度较高,分别占蛋白质总量的55.2%和31.8%;HPLC结果表明,主要金钱白花蛇醇溶蛋白的保留时间为2.928和3.452 min;FTIR结果表明,金钱白花蛇醇溶蛋白在3526.67、2918.31、2357.83、1940.24、1354.05、841.90、546.07和444.52 cm^(-1)处有特征吸收峰。试验结果可为金钱白花蛇醇溶蛋白资源的开发利用及药理活性研究提供依据。Using the Bungarus multicinctus as raw material,the extraction process of gliadin was optimized by response surface method on the basis of single factor test,and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),high performance liquid chromatography(HPLC)and Fourier transform infrared spectroscopy(FTIR)fingerprint analysis were carried out.The results showed that the optimum extraction conditions were as follows:ethanol volume fraction 40%,solid liquid ratio 1∶17(g/mL),extraction time 4 h and extraction temperature 40℃,and the yield ofgliadin was 0.51%.SDS-PAGE fingerprint analysis showed that the molecular weight of gliadin of the Bungarus multicinctus was mainly between 26.69-88.02 kDa,and protein abundance at 26.69-28.34 kDa and 70.84-88.02 kDa were higher,accounting for 55.2%and 31.8%of total protein,respectively.The results of HPLC showed that the retention time of the main gliadin of Bungarus multicinctus was 2.928 and 3.452 min.The results of FTIR showed that there were characteristic absorption peaks at 3526.67,2918.31,2357.83,1940.24,1354.05,841.90,546.07 and 444.52 cm^(-1).The results of this experiment can provide a basis for the development and utilization of the gliadin resources and the study of pharmacological activities of the Bungarus multicinctus.

关 键 词：金钱白花蛇 醇溶蛋白 提取工艺 响应面 优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]