栀子苷与热处理大豆分离蛋白相互作用机理  

Study on the interaction mechanism between Geniposide and heat-treated soybean protein isolate

作　　者：冯思麟 范浩伟 马文婧 赵富强 谢芳 罗竞成 舒佳 付桂明[1,2] 万茵[1] FENG Silin;FAN Haowei;MA Wenjing;ZHAO Fuqiang;XIE Fang;LUO Jingcheng;SHU Jia;FU Guiming;WAN Yin(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;International Institute of Food Innovation,Nanchang University,Nanchang 330047,China;OSMY GROUP CO.,LTD,Nanchang 330038,China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学国际食品创新研究院,江西南昌330047 [3]欧思麦集团有限公司,江西南昌330038

出　　处：《南昌大学学报（理科版）》2023年第1期40-47,共8页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(32060540)。

摘　　要：为探究栀子苷与不同热处理大豆分离蛋白的相互作用的机理,以Zeta电位、粒径及微观样貌观察(SEM)为指标对栀子苷-热处理大豆分离蛋白复合物进行表征,并采用荧光光谱法探究栀子苷与不同热处理大豆分离蛋白之间的淬灭方式、结合位点数、结合作用力类型。结果表明,栀子苷会使热处理大豆分离蛋白的Zeta电位绝对值增大,粒径减小,且栀子苷与80℃热处理大豆分离蛋白的复合物Zeta电位绝对值最大,粒径最小;栀子苷与热处理大豆分离蛋白之间淬灭过程是自发的,机制为静态淬灭,相互作用类型为范德华力和氢键,并形成了结合位点数近似于1的复合物。In order to explore the mechanism of the interaction between Geniposide and soybean protein isolates with different heat treatments, the GE-heat treated SPI complex were characterized by Zeta potential, particle size and microscopic appearance observation, and fluorescence spectroscopy was used to explore the quenching mode, number of binding sites, and types of binding forces between Geniposide and heat-treated soybean protein isolates.The results showed that the absolute value of Zeta potential of heat-treated soybean protein isolate was increased by Geniposide, and the particle size was decreased.Moreover, the absolute value of Zeta potential of the complex of Geniposide and heat-treated soybean protein isolate at 80 ℃ is the largest, and the particle size is the smallest;For van der Waals forces and hydrogen bonds, and form a complex with approximately 1 binding site.

关 键 词：相互作用 大豆分离蛋白 栀子苷 

分 类 号：TS261.1[轻工技术与工程—发酵工程]