CRISPR/Cas系统的核酸检测及其在食品安全快速检测中的应用  

CRISPR/Cas-based Nucleic Acid Detection and Its Application in Rapid Detection for Food Safety

作　　者：翟百强 程楠[3] 王洪涛 徐瑗聪 Zhai Baiqiang;Cheng Nan;Wang Hongtao;Xu Yuancong(Faculty of Environment and Life,Beijing University of Technology,Beijing 100024;Henan Railway Food Safety Management Engineering Technology Research Center,Zhengzhou 450052;College of Food Science&Nutritional Engineering,China Agriculture University,Beijing 100083)

机构地区：[1]北京工业大学环境与生命学部,北京100024 [2]河南省铁路食品安全管理工程技术研究中心,郑州450052 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国食品学报》2023年第2期383-397,共15页Journal of Chinese Institute Of Food Science and Technology

基　　金：中国博士后面上资助项目(2021M690277)。

摘　　要：近年来,快速检测技术成为食品安全领域的一个重要研究方向。成簇规律间隔短回文序列(CRISPR)及CRISPR相关蛋白(Cas)组成的CRISPR/Cas系统因优越的靶向性而被广泛应用于核酸检测中。基于CRISPR/Cas系统的核酸传感器具有灵敏度高、特异性强、时效性快等优势,成为快检领域的研究热点,也推动了食品安全快速检测技术的发展。本文基于CRISPR/Cas系统进行综述,分析不同的CRISPR/Cas系统结合核酸技术在靶标检测中的应用情况,展望CRISPR/Cas核酸传感器在食品安全快速检测领域的未来研究方向,为研发基于此核酸传感器的新型检测方法提供参考。In recent years,rapid detection technology has become one of the most important topics in the scope of food safety.CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated protein(Cas).Due to its superior targetability,CRISPR/Cas system has been widely used in the nucleic acid detection.CRISPR/Cas nucleic acid biosensor has become the research hotspot of rapid detection,because of its high sensitivity,strong specificity and good timeliness,driving the development of rapid detection for food safety.This review summarized the development of CRISPR/Cas system,introducing CRISPR/Cas systems combined with different nucleic acid amplification technologies,and their applications in target detection.Furthermore,the use of CRISPR/Cas nucleic acid biosensors in rapid detection for food safety was discussed,and future research directions was proposed.Overall,this review provides references and ideas for developing novel rapid detection methods utilizing CRISPR/Cas nucleic acid biosensors.

关 键 词：成簇规律间隔短回文序列(CRISPR) 核酸传感器 食品安全 快速检测 

分 类 号：TS207.3[轻工技术与工程—食品科学] TS201.6[轻工技术与工程—食品科学与工程]