星鳗(Astroconger myriaster)多肽体外抗氧化活性和功能特性研究  

Antioxidant Activity and Functional Properties of Polypeptides from Astroconger Myriaster

作　　者：陈鑫阳 谢超 尚子寒 郑霖波 郑炜 CHEN Xinyang;XIE Chao;SHANG Zihan;ZHENG Linbo;ZHENG Wei(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;Zhoushan Changqing Ocean Food Company,Zhoushan 316021,China)

机构地区：[1]浙江海洋大学食品与药学学院,浙江舟山316022 [2]舟山市常青海洋食品有限公司,浙江舟山316021

出　　处：《食品科技》2023年第1期141-147,共7页Food Science and Technology

基　　金：浙江省舟山市重点科技项目(2022C01017)。

摘　　要：为探究星鳗(Astroconger myriaster)多肽的体外抗氧化活性及其功能特性,选择中性蛋白酶对星鳗鱼肉进行水解来初步制备星鳗多肽,对其水解液使用超滤法分离获得星鳗抗氧化肽,并测定其分离前后的星鳗多肽清除DPPH自由基、羟自由基、ABTS自由基能力以及该多肽的还原力和功能特性。结果显示,超滤后制备出的星鳗多肽体外抗氧化活性指标均大于超滤前,在样品浓度为10.0 mg/mL时,DPPH自由基清除率分别为74.3%和63.5%,还原力为1.12和0.93。超滤后的星鳗多肽在不同pH值的影响下,溶解度仍然较高,均高于80%,两者在pH4时的起泡率最高,分别为85.2%和79.1%。制备的2种星鳗多肽在温度为50℃时吸油性最大,达到了5.48 mL/g和4.27 mL/g,实验中前6 h吸水性均上升较快,在第6小时时达到了18.1%和16.5%,在此之后吸水性增长速率逐渐平缓。In order to explore the antioxidant activity and functional properties of Astroconger myriaster peptides in vitro, neutral protease was selected to hydrolyze Astroconger myriaster meat to prepare Astroconger myriaster polypeptides. The antioxidant peptides were isolated from the hydrolysate by ultrafiltration method, and the scavenging ability of DPPH free radical, hydroxyl free radical and ABTS free radical, as well as the reducing power and functional properties of the peptides before and after separation were determined. The results showed that the antioxidant activity indexes of the polypeptides prepared after ultrafiltration were higher than those before ultrafiltration. The DPPH radical scavenging activity and reducing power were 74.3% and 63.5%, respectively, at the concentration of 10.0 mg/mL.Under the influence of different pH values, the solubility of the ultrafiltration peptides was still higher than 80%, and the foaming rate of the two peptides was the highest at pH 4, 85.2% and 79.1%, respectively.When the temperature was 50 ℃, the oil absorbance of the two kinds of polypeptides was maximum,reaching 5.48 mL/g and 4.27 mL/g. In the water absorption experiment, the water absorption increased rapidly in the first 6 hours, and reached 18.1% and 16.5% in the sixth hour. After that, the increase rate of water absorption gradually slowed down.

关 键 词：星鳗 海洋生物活性肽 抗氧化活性 功能性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]