龙眼多糖的结构表征及其激活巨噬细胞的作用  

Structural Characterization of Longan Polysaccharide and Its Activating Effect on Macrophages

作　　者：张静[1] 冯潮 王胜威 杨瑞丽[2] 李武[1,3] ZHANG Jing;FENG Chao;WANG Shengwei;YANG Ruili;LI Wu(College of Food Science and Engineering,Hainan University,Haikou 570228,China;College of Food Science,South China Agricultural University,Guangzhou 510642,China;School of Biotechnology and Health Sciences,Wuyi University,Jiangmen 529020,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570228 [2]华南农业大学食品学院,广东广州510642 [3]五邑大学生物科技与大健康学院,广东江门529020

出　　处：《食品科学》2023年第5期103-111,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(31960481,31360380);广东省自然科学基金项目(2021A1515012135)。

摘　　要：为探究龙眼多糖的结构及其对巨噬细胞的作用,通过分级醇沉的方法从龙眼果肉中分离纯化获得1种均一性较好的多糖组分(记为LP),采用凝胶渗透色谱激光光散射、傅里叶变换红外光谱、核磁共振等方法对LP的结构进行表征,并分析LP对巨噬细胞吞噬能力以及肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-1β和IL-6分泌的影响。结果显示,LP是由葡萄糖(glucose,Glc)、半乳糖、甘露糖、甘露糖醛酸、阿拉伯糖和鼠李糖以物质的量分数比为98.70%∶0.57%∶0.35%∶0.09%∶0.20%∶0.12%组成的重均分子质量为8.42×10^(5) Da的杂多糖,其主链结构为■6)-α-Glcp-(1→6)-α-Glcp-(1■ 4)-α-Glcp-(-1。同时,50、100、200μg/mL的LP具有促进巨噬细胞吞噬,增加TNF-α、IL-1β和IL-6分泌的作用。其中200μg/m L LP诱导巨噬细胞的吞噬率为空白对照组(以完全培养基替代LP)的195.61%;50、100、200μg/m L的LP刺激巨噬细胞后,TNF-α、IL-1β和IL-6分泌量分别为空白对照组的2.38、10.51倍和10.67倍。综上,LP具有激活巨噬细胞的作用,本实验的结果可以为龙眼的精深加工提供科学依据。To explore the structure of longan polysaccharide and its effect on macrophages,a homogenous polysaccharide(named LP)was isolated and purified from longan pulp by ethanol fractional precipitation,and the structure of LP was characterized by gel permeation chromatography with laser light scattering(GPC-LLS),infrared spectroscopy(IR)and nuclear magnetic resonance(NMR)spectroscopy.Meanwhile,its effect on the phagocytic capacity of macrophages and the secretion of tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6.The results showed that LP was a heteropolysaccharide with a weight average molecular mass of 8.42×10^(5) Da composed of glucose,galactose,mannose,mannuronic acid,arabinose and rhamnose at a molar ratio of 98.70%:0.57%:0.35%:0.09%:0.20%:0.12%.NMR spectroscopic analysis showed the backbone chain structure of LP to be■ 6)-α-Glcp-(1→6)-α-Glcp-(1■4)-α-Glcp-(-1.LP at 50,100,200μg/mL promoted the phagocytic capacity of macrophages and increased the secretion of TNF-α,IL-1βand IL-6.The phagocytic capacity of LP-induced macrophages increased by 95.61%compared with the untreated control group.TNF-α,IL-1βand IL-6 secretion in macrophages challenged by LP at 50,100 and 200μg/mL were 2.38,10.51 and 10.67 times higher than those in the control group,respectively.These results showed that LP had an activating effect on macrophages,which can provide a scientific basis for the deep processing of longan fruit.

关 键 词：龙眼 多糖 纯化 结构表征 巨噬细胞 

分 类 号：TS201.4[轻工技术与工程—食品科学]