Targeting macrophagic 17β-HSD7 by fenretinide for the treatment of nonalcoholic fatty liver disease  

作　　者：Xiaoyu Dong Yiting Feng Dongqin Xu Mengya Zhang Xiao Wen Wenhao Zhao Qintong Hu Qinyong Zhang Hui Fu Jie Ping 

机构地区：[1]Department of Pharmacology,School of Basic Medical Sciences,Wuhan University,Wuhan 430071,China [2]Department of Anatomy,School of Basic Medical Sciences,Wuhan University,Wuhan 430071,China

出　　处：《Acta Pharmaceutica Sinica B》2023年第1期142-156,共15页药学学报（英文版）

基　　金：supported by the National Nature Science Foundation of China(Nos.82173872 and 81872663)。

摘　　要：Nonalcoholic fatty liver disease(NAFLD)is the most common chronic liver disease worldwide and macrophage polarization plays an important role in its pathogenesis.However,which molecule regulates macrophage polarization in NAFLD remains unclear.Herein,we showed NAFLD mice exhibited increased 17β-hydroxysteroid dehydrogenase type 7(17β-HSD7)expression in hepatic macrophages concomitantly with elevated M1 polarization.Single-cell RNA sequencing on hepatic non-parenchymal cells isolated from wild-type littermates and macrophage-17β-HSD7 knockout mice fed with high fat diet(HFD)for 6 weeks revealed that lipid metabolism pathways were notably changed.Furthermore,17β-HSD7 deficiency in macrophages attenuated HFD-induced hepatic steatosis,insulin resistance and liver injury.Mechanistically,17β-HSD7 triggered NLRP3 inflammasome activation by increasing free cholesterol content,thereby promoting M1 polarization of macrophages and the secretion of pro-inflammatory cytokines.In addition,to help demonstrate that 17β-HSD7 is a potential drug target for NAFLD,fenretinide was screened out from an FDA-approved drug library based on its 17β-HSD7 dehydrogenase inhibitory activity.Fenretinide dose-dependently abrogated macrophage polarization and pro-inflammatory cytokines production,and subsequently inhibited fat deposition in hepatocytes co-cultured with macrophages.In conclusion,our findings suggest that blockade of 17β-HSD7 signaling by fenretinide would be a drug repurposing strategy for NAFLD treatment.

关 键 词：Nonalcoholic fatty liver disease 17β-HSD7 MACROPHAGE CHOLESTEROL Insulin resistance FENRETINIDE 

分 类 号：R575.5[医药卫生—消化系统]