Actively separated microneedle patch for sustained-release of growth hormone to treat growth hormone deficiency  

作　　者：Li Yang Qingyun Liu Xinhui Wang Nansha Gao Xiuzhen Li Hongzhong Chen Lin Mei Xiaowei Zeng 

机构地区：[1]Institute of Pharmaceutics,School of Pharmaceutical Sciences(Shenzhen),Sun Yat-sen University,Shenzhen 518107,China [2]Department of Genetics and Endocrinology,Guangzhou Women and Children's Medical Center,Guangzhou 510623,China [3]Tianjin Key Laboratory of Biomedical Materials,Key Laboratory of Biomaterials and Nanotechnology for Cancer Immunotherapy,Institute of Biomedical Engineering,Chinese Academy of Medical Sciences,Peking Union Medical College,Tianjin 300192,China

出　　处：《Acta Pharmaceutica Sinica B》2023年第1期344-358,共15页药学学报（英文版）

基　　金：financial support from the National Natural Science Foundation of China(32071342 and 31922042);Guangdong Special Support Program(2019TQ05Y209);the Natural Science Foundation of Guangdong Province(2021A1515010431);the Fundamental Research Funds for the Central Universities(Nos.2021-RC310-005,2020-RC320-002 and 2019PT320028);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-058)。

摘　　要：Growth hormone deficiency(GHD)has become a serious healthcare burden,and presents a huge impact on the physical and mental health of patients.Here,we developed an actively separated microneedle patch(PAA/NaHCO_(3)^(-)Silk MN)based on silk protein for sustained release of recombinant human growth hormone(rhGH).Silk protein,as a friendly carrier material for proteins,could be constructed in mild full-water conditions and ensure the activity of rhGH.After manually pressing PAA/NaHCO_(3)^(-)Silk MN patch to skin for 1 min,active separation is achieved by absorbing the interstitial fluid(ISF)to trigger HCO_(3)^(-)in the active backing layer to produce carbon dioxide gas(CO_(2)).In rats,the MN patch could maintain the sustained release of rhGH for more than 7 days,and produce similar effects as daily subcutaneous(S.C.)injections of rhGH in promoting height and weight with well tolerated.Moreover,the PAA/NaHCO_(3)^(-)Silk MN patch with the potential of painless self-administration,does not require cold chain transportation and storage possess great economic benefits.Overall,the PAA/NaHCO_(3)^(-)Silk MN patch can significantly improve patient compliance and increase the availability of drugs,meet current unmet clinical needs,improve clinical treatment effects of GHD patients.

关 键 词：MICRONEEDLE Silk protein SELF-ADMINISTRATION SUSTAINED-RELEASE Actively separated Growth hormone deficiency Growth hormone Long-acting GH 

分 类 号：R58[医药卫生—内分泌]