淡紫拟青霉固态发酵黄芩药渣工艺优化  

Optimization of solid-state fermentation process of Scutellaria baicalensis residue by Paecilomyces lilacinus

作　　者：甄静[1] 武艳芳 王继雯[1] 李磊 向凌云[1] 李亮亮 杨文玲[1] ZHEN Jing;WU Yanfang;WANG Jiwen;LI Lei;XIANG Lingyun;LI Liangliang;YANG Wenling(Institute of Biology Co.,Ltd.,Henan Academy of Sciences,Zhengzhou 450008,China;Palm Eco-Town Development Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008 [2]棕榈生态城镇发展股份有限公司,河南郑州450000

出　　处：《中国酿造》2023年第3期169-173,共5页China Brewing

基　　金：国家自然科学基金项目(31800361);河南省科学院科技开放合作项目(210905018);河南省科学院重大科研聚焦专项(210105002)。

摘　　要：为了降低淡紫拟青霉(Paeciloids lilacinus)的固态发酵成本,以黄芩药渣废弃物为基质,对其培养基组成及发酵条件进行优化,并考察不同几丁质含量对菌株产几丁质酶活性的影响。结果表明,淡紫拟青霉固态发酵的最佳培养基组成为黄芩药渣20 g,玉米粉0.8 g,尿素0.02 g,含水量80%,固态发酵最佳的培养条件为培养温度28℃,接种量10%,培养时间7 d,在此优化条件下,分生孢子数可达8.98×10^(9) CFU/g。添加0.9%几丁质时,可显著增加菌株产几丁质酶酶活性(P<0.05)。In order to reduce the cost of solid-state fermentation of Paeciloids lilacinus,using Scutellaria baicalensis residue as the substrate,the medium composition and fermentation conditions were optimized,and the effects of different chitin content on chitinase-producing activity were investigated.The results showed that the optimal medium composition for solid-state fermentation of P.lilacinus was as follows:S.baicalensis residue 20 g,corn flour 0.8 g,urea 0.02 g,and water content 80%.The optimum culture conditions of solid-state fermentation were as follows:culture temperature 28℃,inoculum 10%,and time 7 d.Under the optimal condition,the conidia number could reach 8.98×10^(9) CFU/g.The chitinase-producing activity of the strain was significantly increased when the chitin addition was 0.9%(P<0.05).

关 键 词：淡紫拟青霉 黄芩药渣 固态发酵 工艺优化 

分 类 号：TQ458.1[化学工程—农药化工]