响应面优化双酶法提取云南滇黄精水不溶性膳食纤维工艺  

Process Optimization of Double Enzymatic Extraction Technology of Insoluble Dietary Fiber from Polygonatum Kingianum by Response Surface Methodology

作　　者：姚波 李冬丽 郭茵 谭艳霞 YAO Bo;LI Dongli;GUO Yin;TAN Yanxia(Faculty of Environmental and Chemical Engineering,Kunming Metallurgy College,Kunming 650033,China)

机构地区：[1]昆明冶金高等专科学校环境与化工学院,云南昆明650033

出　　处：《昆明冶金高等专科学校学报》2022年第6期59-66,共8页Journal of Kunming Metallurgy College

基　　金：昆明冶金高等专科学校科研基金项目:利用云南滇黄精药渣制备膳食纤维的研究(2020XJZK02)。

摘　　要：为促进滇黄精渣的综合利用,以云南滇黄精药渣为原料,利用淀粉酶和蛋白酶提取药渣中的水不溶性膳食纤维(IDF)。通过单因素试验,研究加酶量、pH和温度对滇黄精IDF提取率的影响;采用Box-Behnken响应面分析法优化确定提取滇黄精IDF的工艺条件。结果显示:滇黄精水不溶性膳食纤维的最优提取工艺为α-淀粉酶的添加量176 U/g、酶解pH 6、酶解温度56℃;碱性蛋白酶的添加量6 100 U/mg、酶解pH 9、酶解温度55℃。在此条件下,滇黄精IDF平均提取率可达62.86%,IDF的持水力为9.76 g/g,持油力为5.74 g/g,膨胀力为5.86 mL/g。该工艺条件稳定可靠,可为提升滇黄精副产物的附加值及开发保健食品提供参考。In order to promote the comprehensive utilization of Polygonatum kingianum residue,the insoluble dietary fiber(IDF)from Polygonatum kingianum residue in Yunnan Province was extracted by amylase and protease.The effects of the amount of enzyme,pH and temperature on the extraction rate of dietary fiber were studied by single factor experiment.The Box-Behnken response surface analysis was used to optimize the extraction conditions of dietary fiber from Polygonatum kingianum residue.The results showed:theα-amylase addition amount was 176 U/g,the enzymatic hydrolysis pH was 6,the enzymatic hydrolysis temperature was 56℃;the alkaline protease addition amount was 6100 U/mg,the enzymatic hydrolysis pH was 9,the enzymatic hydrolysis temperature was 55℃.Under this condition,the average extraction yield of IDF was 62.86%,the water holding capacity of IDF was 9.76 g/g,the oil holding capacity was 5.74 g/g,and the expansion capacity was 5.86 mL/g.The extraction process was stable and reliable,which provided a reference forimproving the value of Polygonatum kingianum by product and developing of health food.

关 键 词：滇黄精药渣 水不溶性膳食纤维 响应面 双酶 工艺优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]