超高效液相色谱串联质谱法测定乳制品中透明质酸  

Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：杜国辉[1] 范维江[1] 陈玉娟 陈雯雯 乔莉苹 王萃玲 DU Guohui;FAN Weijiang;CHEN Yujuan;CHEN Wenwen;QIAO Liping;WANG Cuiling(Department of Food Industry,Shandong Institute of Commerce and Technology,Jinan 250103,China;Bloomage Biotechnology Corporation Limited,Jinan 250101,China;Qilu Medical University,Jinan 250103,China)

机构地区：[1]山东商业职业技术学院食品工业产业学院,山东济南250103 [2]华熙生物科技股份有限公司,山东济南250101 [3]齐鲁医药学院,山东济南250103

出　　处：《食品工业科技》2023年第8期334-340,共7页Science and Technology of Food Industry

摘　　要：本文建立一种超高效液相色谱-串联质谱(UPLC-MS/MS)测定乳制品中透明质酸含量的分析方法。样品经透明质酸酶降解,乙腈稀释,PRIME HLB固相萃取柱净化,Waters BEH Amide(2.1 mm×100 mm,1.7μm)酰胺柱分离,以0.2%氨水-乙腈(含0.2%氨水)为流动相梯度洗脱。采用电喷雾离子源负离子模式扫描,多反应监测模式进行检测,外标法定量。结果表明:乳制品中透明质酸在0.5~200 mg/kg添加水平下回收率为91.4%~106.2%,RSD为2.3%~6.7%。方法的定量限为0.5 mg/kg。该方法简单,适用于乳制品中透明质酸的定量检测。应用本方法对市售乳制品中透明质酸进行检测,所测样品符合标准要求。The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry(UPLC-MS/MS)for determining hyaluronic acid in dairy products was proposed in this study.The samples were degraded by hyaluronidase,diluted with acetonitrile,and then purified by PRIME HLB cartridge.The analysis was conducted on a BEH Amide column(2.1 mm×100 mm,1.7μm)by gradient elution using 0.2%concentration of aqueous ammonia solution and acetonitrile(containing 0.2%ammonia)as mobile phases.Qualification was achieved by negative electrospray ionization with multiple reaction monitoring(MRM).Meanwhile,the external standard method for quantitation was applied.In accordance with the results,the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%,and RSDs were 2.3%~6.7%at the addition levels of 0.5~200 mg/kg.Presumably,with a limit of quantification of 0.5 mg/kg,the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products.This method was used to detect commercially dairy products,and the tested samples meet the standard requirements.

关 键 词：超高效液相串联质谱法 乳制品 透明质酸 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]