一种高纯度小分子解酒护肝肽的制备  

Preparation of a high purity small molecule anti-alcoholic liver protecting peptide

作　　者：温庆仕 魏荷芬 周精卫 应汉杰 陈勇[1,2,3] 刘庆国 袁童[2] 刘淼 沈才洪 王松涛[4] WEN Qingshi;WEI Hefen;ZHOU Jingwei;YING Hanjie;CHEN Yong;LIU Qingguo;YUAN Tong;LIU Miao;SHEN Caihong;WANG Songtao(Jiangsu Jicui Industrial Biotechnology Research Institute Co.Ltd.,Nanjing 211800,China;Nanjing Hi Tech University Biotechnology Research Institute Co.Ltd.,Nanjing 211800,China;National Biochemical Engineering Technology Research Center,Nanjing University of Technology,Nanjing 210000,China;Luzhou Laojiao Co.Ltd.,Luzhou 646000,China)

机构地区：[1]江苏集萃工业生物技术研究所有限公司,江苏南京211800 [2]南京高新工大生物技术研究院有限公司,江苏南京211800 [3]南京工业大学国家生化工程技术研究中心,江苏南京210000 [4]泸州老窖股份有限公司,四川泸州646000

出　　处：《食品与发酵工业》2023年第7期174-180,共7页Food and Fermentation Industries

基　　金：江苏省科协技术基金项目(BE2019001)。

摘　　要：目前海洋生物活性肽在功能食品中的应用已成为研究热点。针对中西药类解酒产品不足的问题,该研究制备了一种高纯度、高活性的小分子解酒护肝肽产品。采用多酶中度水解鱿鱼蛋白,以蛋白质回收率和乙醇脱氢酶(alcohol dehydrogenase,ADH)激活率为指标。确定了最佳酶解条件为胃蛋白酶∶碱性蛋白酶∶风味蛋白酶=1∶1∶1(质量比),酶添加量为0.2%(质量分数)、料液比为1∶10(g∶mL)、反应时间3 h。在最佳酶解条件下的酶解液,其蛋白回收率为80.34%,ADH激活率为83.37%。为进一步提纯小肽并提升其功能活性,对酶解液进行精制工艺研究,采用树脂吸附疏水性肽组分,用梯度乙醇洗脱,收集液浓缩、干燥,获得淡黄色产品肽粉末。其蛋白肽纯度为95.33%,其分子质量<3000 Da的占99.96%,对ADH的酶活率为122.71%,对DPPH自由基的清除率可达92.01%。该研究制备的小肽产品在功能食品领域具有广阔的应用前景。At present,the research of marine bioactive peptides in functional foods has become popular.To solve the problem of the deficiency in traditional Chinese and Western anti-alcoholics,a small molecule anti-alcoholic liver protecting peptide with high purity and activity was prepared in this study.Squid protein was moderately hydrolyzed by multiple enzymes,and the protein recovery rate and alcohol dehydrogenase(ADH)activation rate were used as indicators.The optimum enzymatic hydrolysis conditions were∶pepsin∶alkaline protease∶flavor protease=1∶1∶1,enzyme addition 0.2%(mass fraction),solid-liquid ratio 1∶10(g∶mL)and reaction time 3 h.Under the optimum conditions,the protein recovery rate was 80.34%and the ADH activation rate was 83.37%.In order to further purify the small peptide and improve its functional activity,the refining process of the enzymatic hydrolysate was studied.The hydrophobic peptide components were adsorbed by resin,eluted with gradient ethanol,and the collected solution was concentrated and dried to obtain a light yellow peptide powder.The purity of its protein peptide was 95.33%,peptide with the molecular weight under 3000 Da was 99.96%,the ADH activation ratio was 122.71%,and the scavenging rate of DPPH free radical was 92.01%.The small peptide products prepared in this study have great application potential in the field of functional food.

关 键 词：多酶中度水解 小分子肽 ADH激活率 DPPH自由基 解酒护肝 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]