PU.1 interacts with KLF7 to suppress differentiation and promote proliferation in chicken preadipocytes  

作　　者：Ming Tan Hu Xu Jinwei Li Ziqiu Jia Xin Zhang Shuli Shao Weiwei Zhang Weiyu Wang Yingning Sun 

机构地区：[1]College of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar 161003,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第1期143-153,共11页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.31402061);the Heilongjiang Province Natural Science Foundation of China(No.YQ2019C025);the Project of the Basic Business of Heilongjiang Provincial Education Department(No.145109211).

摘　　要：Krüppel-like factor 7(KLF7)is a negative regulator of preadipocyte differentiation.Our previous KLF7 ChIP-seq analysis showed that the binding motif of PU.1 was found among the KLF7 binding peaks,indicating that an interaction between KLF7 and PU.1 at preadipocyte gene promoters and other regulatory elements might be common.Here,Co-IP and FRET assays are used to confirm that PU.1 can directly bind to KLF7 and enhance the transcription activity of cyclin-dependent kinase inhibitor 3(CDKN3),which is a downstream target gene of KLF7.We show that the PU.1 expression level is decreased during preadipocyte differentiation.Furthermore,PU.1 overexpression and knockdown experiments reveal that PU.1 negatively regulates chicken preadipocyte differentiation,as evidenced by appropriate changes in lipid droplet accumulation and altered expressions of PPARγ,FAS,and PLIN.In addition,PU.1 overexpression promotes preadipocyte proliferation,while knockdown of PU.1 inhibits preadipocyte proliferation.We further demonstrate that PU.1 inhibits differentiation and promotes proliferation in preadipocytes,in part by directly interacting with KLF7.

关 键 词：PU.1 PREADIPOCYTE KLF7 DIFFERENTIATION PROLIFERATION 

分 类 号：R589.2[医药卫生—内分泌]