侧向流免疫检测食品中单增李斯特氏菌的研究进展  

Research Progress in Lateral Flow Immunoassay for Detection of Listeria monocytogenes in Food

作　　者：郦娟 杨永 戴小芳 陈琼 晏涛 熊杰 LI Juan;YANG Yong;DAI Xiao-fang;CHEN Qiong;YAN Tao;XIONG Jie(Wuhan Institute for Food and Cosmetic Control,Wuhan 430000,Hubei,China;Key Laboratory of Edible Oil Quality and Safety for State Market Regulation,Wuhan 430000,Hubei,China)

机构地区：[1]武汉食品化妆品检验所,湖北武汉430000 [2]国家市场监管重点实验室(食用油质量与安全),湖北武汉430000

出　　处：《食品研究与开发》2023年第8期219-224,共6页Food Research and Development

基　　金：国家市场监督管理总局科技项目(2020MK131);湖北省市场监督管理局技术保障和科技计划项目(Hbscjg-JS2021007)。

摘　　要：单增李斯特氏菌监测对于保护食品供应及公众健康至关重要。侧向流免疫测定操作简单、成本低、快速,已被广泛应用于致病菌的即时检测。该文从样品制备和测定两方面概述应用于食品中单增李斯特氏菌检测的侧向流免疫测定检测方案,重点介绍核酸侧向流免疫测定。等温核酸侧向流免疫测定结合其他富集和信号检测方式,有望成为单增李斯特氏菌即时检测研发的新方向。The surveillance of Listeria monocytogenes is critical for protecting the food supply and public health.Lateral flow immunoassay,being simple,cost-saving,and fast,has been widely used in the real-time detection of pathogens.This paper summarizes the schemes of lateral flow immunoassay applied to the detection of L.monocytogenes in food from two aspects of sample preparation and determination,with emphasis on nucleic acid-based lateral flow immunoassay.The isothermal nucleic acid-based lateral flow immunoassays combined with other enrichment and signal detection methods are expected to become a new direction of the real-time detection of L.monocytogenes.

关 键 词：单增李斯特氏菌 食品 侧向流免疫测定 富集 信号检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]