胶体磨复合酶法浸提香菇多糖的工艺优化  

Optimization of Extraction Technology of Lentinan by Colloid Mill Combined with Enzyme

作　　者：唐坤 龙欢 尚校兰[1,2,3] TANG Kun;LONG Huan;SHANG Xiaolan(College of Life Science,Langfang Normal University,Langfang 065000;Technology Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province,Langfang 065000;Langfang Key Laboratory of Food Nutrition and Safety,Langfang 065000)

机构地区：[1]廊坊师范学院生命科学学院,廊坊065000 [2]河北省食药用菌资源高值利用技术创新中心,廊坊065000 [3]廊坊市食品营养与安全重点实验室,廊坊065000

出　　处：《食品工业》2023年第3期86-90,共5页The Food Industry

基　　金：廊坊师范学院校科研基金—博士科研启动项目(XBQ202032)。

摘　　要：优化胶体磨复合酶法浸提香菇中香菇多糖的工艺。以香菇多糖提取率为评价指标,采用胶体磨复合酶法,经单因素试验、正交试验和工艺验证,优化其关键工艺参数并比较。结果显示,优化后的参数为料液比1︰40 (g/m L)、酶添加量2.5%、酶解温度50℃、酶解时间40 min。在此条件下香菇多糖提取率为21.59%,提取效率比其他提取方法提高1.3~3.3倍(P<0.05)。该工艺节能环保、条件温和,为香菇多糖的产业化优化提供参考。To optimize the extraction process of lentinan from Lentinus edodes by colloid mill combined with enzyme,with the extraction rate of lentinan as the evaluation index,the key process parameters of lentinan were optimized and compared through single factor test,orthogonal test and process validation by using colloid mill compound enzyme method.The results showed that the optimized parameters were as follows:the liquid ratio 1:40(g/mL),the amount of enzyme added 2.5%,the temperature of enzymatic hydrolysis 50℃,and the time of enzymatic hydrolysis 40 min.Under this condition,the extraction rate of Lentinus edodes polysaccharide was 21.59%,and the extraction efficiency was 1.3-3.3 times higher than that of other extraction methods(P<0.05).The process is energy saving,environmental protection and mild conditions,which will provide a new reference for the optimization of lentinan industrialization.

关 键 词：香菇多糖 胶体磨 复合酶 浸提 工艺优化 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]