黑莓籽油提取工艺及功能应用研究  

Research on Extraction Technology and Functional Application of Blackberry Seed Oil

作　　者：赵俸艺 王邵熠 葛俊高 樊苏帆 吴文龙[1] 李维林 ZHAO Feng-yi;WANG Shao-yi;GE Jun-gao;FAN Su-fan;WU Wen-long;LI Wei-lin(Fruit Research Center Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem,Sun Yat-Sen)/Jiangsu Key Laboratory for the Research and Utilization of Plant Resourses,Nanjing 210014,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,China;Xinghua Forestry Technical Guidance Station,Xinghua 225700,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)果树研究中心/江苏省植物资源与利用重点实验室,江苏南京210014 [2]南京林业大学林学院,江苏南京210037 [3]兴化市林业技术指导站,江苏兴化225700

出　　处：《江西农业学报》2023年第2期187-193,共7页Acta Agriculturae Jiangxi

基　　金：江苏省科技厅现代农业重点面上项目(BE2020344);江苏省中国科学院植物研究所博士人才科研启动基金项目(JIBTF 202108)。

摘　　要：采用超声辅助溶剂萃取法制备了黑莓籽油,并采用MTT法研究了该黑莓籽油对宫颈癌细胞(Hela)、肝癌细胞(HepG2)、乳腺癌细胞(MCF-7)、非小细胞肺癌细胞(A549)和人脐静脉表皮细胞(HUVEC)的抑制增殖活性。该制备方法易操作,试验中所需物品无毒,并发现黑莓籽油对上述4种癌细胞的IC_(50)值分别为176.99、172.18、206.50和413.94μg/mL,而对正常细胞(HUVEC)的IC_(50)值>500μg/mL,表明该黑莓籽油抑制Hela细胞、HepG2细胞、MCF-7细胞和A549细胞增殖活性的作用较强,且无毒,为抗癌药的开发提供了新思路,拓宽了在绿色植物中挖掘新油脂原料的来源;并且该黑莓种籽油突破了传统食用油的局限性,是一类具有优良前景的高级保健食用油。The blackberry seed oil was prepared by ultrasonic-assisted solvent extraction,which was non-toxic and easy to implement.MTT assay was used to study proliferation inhibition(IC_(50))of the blackberry seed oil on cervical cancer cells(Hela),liver cancer cells(HepG2),breast cancer cells(MCF-7),pulmonary adenocarcinoma cells(A549)and human umbilical vein endothelial cells(HUVEC).The IC_(50)values were 176.99μg/mL,172.18μg/mL,206.50μg/mL and 413.94μg/mL against Hela,HepG2,MCF-7 and A549 cells respectively,indicating that extracted blackberry seed oil had good inhibitory activity against the proliferation of Hela,HepG2,MCF-7 and A549 cells.The normal cells were also used to prove the non-toxicity of blackberry seed oil,which named human umbilical vein endothelial cells(HUVEC).The IC_(50)value was more than 500μg/mL,suggesting the prepared blackberry seed oil were non-toxic.The study provides a new idea for the development of anticancer drugs,and broadening the source of new oil materials in natural plants.The blackberry seed oil is a kind of advanced health edible oil with good prospects,which breaks through the limitation of traditional edible oil.

关 键 词：黑莓籽油 提取工艺 抑制增殖 保健食用油 

分 类 号：TS261.9[轻工技术与工程—发酵工程]