Effect of micro/nano-sheet array structures on the osteo-immunomodulation of macrophages  

作　　者：Xinhui Zheng Lan Chen Ji Tan Jianhua Miao Xuanyong Liu Tieyi Yang Zhihong Ding 

机构地区：[1]Department of Orthopedics,Gongli Hospital of Shanghai Pudong New Area,Shanghai 200135,China [2]Department of Orthopedics,Zhengzhou Central Hospital Affiliated Zhengzhou University,Zhengzhou 450007,China [3]State Key Laboratory of High-Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China [4]School of Materials Science and Engineering&Henan Key Laboratory of Advanced Magnesium Alloy&Key Laboratory of Materials Processing and Mold Technology(Ministry of Education),Zhengzhou University,Zhengzhou 450001,China

出　　处：《Regenerative Biomaterials》2022年第1期888-899,共12页再生生物材料（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(51831011,31870944);Medical discipline Construction Project of Pudong Health Committee of Shanghai(PWYts2021-05);Postdoctoral Science Foundation of China(2021 M693260).

摘　　要：The immune response induced by surface topography crucially determines the implant success.However,how the immune response is mediated by the size of surface topography remains unclear.Hence,various biocompatible Mg-Al layered double hydroxides sheet-array films with different sizes(nano,micro and nano/micro mixture)were constructed on the biomedical titanium,and their osteo-immunomodulation effects on the macrophages were explored.The nano-sheet array structures significantly promoted the polarization of M2 macrophages by activating the PI3K-AKT-mTOR signaling pathway with high gene expressions of integrin b2 and FAK.While the micro-sheet array structures enhanced osteogenic differentiation of mouse bone marrow mesenchymal stem cells(mBMSCs)via ROCK-YAP/TAZ-mediated mechanotransduction.Moreover,the nano-sheet array structures promoted the osteogenic differentiation of mBMSCs with a high proportion of M2 macrophages through a shared medium.This study gave further information concerning integrin-induced focal adhesions in cells of different sheet array structures and their role in macrophage polarization and osteogenic differentiation of mBMSCs,which might help to design biomaterial surfaces with optimal geometry for a desired immunemodulation.

关 键 词：micro-nano topography IMMUNOMODULATION MACROPHAGES OSTEOGENESIS 

分 类 号：R318.08[医药卫生—生物医学工程]