枯草芽孢杆菌固态高密度发酵工艺条件的优化  

Optimization of Solid-state High-density Fermentation Conditions of Bacillus subtilis

作　　者：刘文健 孙慕白 郭丹丹 董欣 牛红红[2] 李达[2] 华梅 迟燕平[2] 苏颖[2] 苗欣宇 王景会[2] LIU Wenjian;SUN Mubai;GUO Dandan;DONG Xin;NIU Honghong;LI Da;HUA Mei;CHI Yanping;SU Ying;MIAO Xinyu;WANG Jinghui(College of Life Sciences,Jilin Normal University,Siping 136000,Jilin China;Institute of Agricultural Products Processing,Jilin Academy of Agricultural Sciences,Changchun 130033,China;Xing'an League Center for Disease Control and Prevention,Ulanhot 137499,Inner Mongolia China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林省农业科学院农产品加工研究所,长春130033 [3]兴安盟疾病预防控制中心,内蒙古乌兰浩特137499

出　　处：《饲料博览》2023年第2期24-29,共6页Feed Review

基　　金：吉林省农业科学院创新工程项目-人才基金(CXGC2021RCY036)。

摘　　要：为提高农副产品的利用率和经济价值,将豆粕、稻壳和玉米秸秆制成固态发酵培养基,并采用单因素试验及四因素三水平的正交试验方法优化了枯草芽孢杆菌固态发酵的最佳发酵工艺。结果表明:固态发酵的最佳工艺参数为原料粒径40目,发酵温度37℃,菌液接种量12%,料液比1:1,此条件发酵后的活菌数可达1.24×10^(9)cfu·g^(-1)。In order to improve the utilization rate and economic value of agricultural by-products,soybean meal,rice husk and straw were made into the solid-state fermentation medium.The best fermentation technology of Bacillus subtilis was optimized by single factor test and orthogonal test of four factors and three levels.Results showed that the optimum process parameter of solid fermentation was 40 mesh at 37℃,the inoculum volume of bacterial solution was 12%and the material-liquid ratio was 1􀏑1.Under this condition,the number of viable bacteria in solid-state fermentation of Bacillus subtilis reached 1.24×10_(9) cfu·g^(-1).

关 键 词：枯草芽孢杆菌 固态发酵 发酵工艺优化 

分 类 号：S816.3[农业科学—饲料科学]